Insight into cold-active xylanase production and xylan degradation pathways in psychrotrophic Acinetobacter sp. HC4 from
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ORIGINAL RESEARCH
Insight into cold-active xylanase production and xylan degradation pathways in psychrotrophic Acinetobacter sp. HC4 from the cold region of China Hailian Zang . Xiaopeng Du . Jinming Wang . Yi Cheng . Yue Wang . Shanshan Sun . Xinyue Zhao . Dapeng Li . Huan Zhang . Chunyan Li
Received: 14 January 2020 / Accepted: 12 June 2020 Ó Springer Nature B.V. 2020
Abstract Hemicellulose is one of the three main components of cornstalk, and xylan is the primary component of hemicellulose. Current research on these materials is mostly concentrated on mesophilic or thermophilic microorganisms that degrade xylan. However, the low temperature and dry climate of cold regions limit the rapid biodegradation of xylan and have become limiting factors in cornstalk resource utilization. This study reports a psychrotrophic bacterial strain isolated from soil in northeast China and
Hailian Zang and Xiaopeng Du contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03286-4) contains supplementary material, which is available to authorized users.
identified as Acinetobacter sp. HC4, which can produce xylanase that is active at low temperatures. To increase the production of this xylanase, fermentation conditions were optimized using the BoxBehnken design of the response surface methodology. The maximal xylanase activity occurred at 15.4 °C and pH 8.39 with a xylan concentration of 12.8 g L-1 and an incubation time of 65.5 h. In addition to xylose and xylooligosaccharides, 19 compounds were detected by HPLC, GC–MS and FTIR during the xylan degradation process mediated by psychrotrophic Acinetobacter sp. HC4. Furthermore, based on these data, several possible xylan degradation pathways in strain HC4 were proposed to help clarify the mechanisms of xylan biodegradation. The results of this study not only describe a valuable psychrotrophic xylan-degrading strain but also offer meaningful information for further investigation of the microbial degradation mechanism of cornstalk in cold regions.
H. Zang J. Wang Y. Wang S. Sun X. Zhao D. Li H. Zhang C. Li (&) College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, People’s Republic of China e-mail: [email protected] X. Du The Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, Guangdong, People’s Republic of China Y. Cheng College of Science, China Agricultural University, Beijing 100083, People’s Republic of China
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Cellulose
Graphic abstract
Keywords Xylan Psychrotrophic xylan-degrading bacterium Cornstalk Cold-active xylanase Biodegradation pathway
Introduction Cornstalk, a potential source of fuel, chemicals, and materials, is an abundant renewable biomass resource but requires efficient conversion to achieve commercial viability (Henson et al. 2018; Ren et al. 2019a). The agricultural fields in the northeast region of China produce large qua
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