Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarb
- PDF / 933,331 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 27 Downloads / 216 Views
Acinetobacter mesopotamicus sp. nov., Petroleum‑degrading Bacterium, Isolated from Petroleum‑Contaminated Soil in Diyarbakir, in the Southeast of Turkey Ömer Acer1 · Kemal Güven2 · Annarita Poli3 · Paola Di Donato3,4 · Luigi Leone3 · Lorena Buono3 · Reyhan Gül Güven5 · Barbara Nicolaus3 · Ilaria Finore3 Received: 28 February 2019 / Accepted: 15 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A new petroleum-degrading bacterium, designated strain GC2T, was isolated from Bozkuş 1 petroleum station in Diyarbakir, located in the southeast of Turkey. Cells were Gram-negative staining, aerobic, coccoid-rods, non-motile, non-spore-forming. The bacterium was found to degrade 100% of n-alkanes ranging from C11 to C34 presented in the 1% crude oil after incubation of 7 days. The membrane phospholipids were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), phosphatidylglycerol (PG), dipalmitoyl-sn-glycerol 1- phosphocholine (PC1), 1,2 dipalmitoyl-sn-glycero-3-phosphocholine monohydrate (PC3), cardiolipin also called diphosphatidylglycerol (CL) and l-α- phosphatidic acid, dipalmitoyl (AP); predominant respiratory ubiquinone was Q-8 and C16:0, C18:1ω9c and C16:1 were the major cellular fatty acids. The 16S rRNA sequence analysis revealed that the strain GC2T was a member of genus Acinetobacter and was most closely related to Acinetobacter lwoffii DSM 2403 T (99.79%), Acinetobacter pseudolwoffii ANC 5318 T (98.83%) and Acinetobacter harbinensis HITLi 7 T (98.14%). The rpoB and gyrB gene sequence analysis confirmed that the strain GC2T was a member of genus Acinetobacter and that the closest relative was Acinetobacter lwoffii DSM 2 403 T (99.08% and 100% similarity, respectively). DNA-DNA hybridization values between GC2T and its closest relatives ranged from 65.6% (with A. lwoffii) to 5.1% (with A. venetianus). The whole genome sequence of strain G C2T was obtained. The DNA G + C content of this strain was determined to be 42.9 mol %. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain G C2T represents an independent genomospecies. On the basis of phenotypic characteristics, chemotaxonomic, phylogenetic data and DNA–DNA hybridization and whole genome analysis, we propose to assign strain G C2T as a new species of the genus Acinetobacter, for which the name Acinetobacter mesopotamicus sp. nov. is proposed. The type strain of this species is GC2T (DSM 26953 T = JCM 31073 T). The whole genome of strain GC2T has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000. Abbreviations ESI/MS Electrospray ionization-mass spectrometry FAMEs Fatty acid methyl esters New Taxa‑ Proteobacteria The GenBank/EMBL/DDBJ accession number for the 16S rRNA, gyrB and rpoB gene sequences of strain GC2T are KJ867435, LT745873 and LT745874, respectively. The whole genome of strain GC2T has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000. Electronic supplementary material The online version of this article (htt
Data Loading...
