Proteomic insights into Lysinibacillus sp.-mediated biosolubilization of manganese
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EMERGING TRENDS IN BIOTECHNOLOGY FOR SUSTAINABLE DEVELOPMENT AND POLLUTION PREVENTION
Proteomic insights into Lysinibacillus sp.-mediated biosolubilization of manganese Shreya Ghosh 1 & Mayuri Gandhi 2 & Eric D. van Hullebusch 3 & Alok Prasad Das 4 Received: 17 June 2020 / Accepted: 14 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract There has been alarming depletion of manganese (Mn) reserves owing to the ongoing extensive mining operations for catering the massive industrial demand of this element. Moreover, the mining operations have been leading to the generation of Mn-rich waste, thereby contaminating both terrestrial and aquatic bodies. The current scenario necessitates the development of alternative processes for bioremediation as well as economic recovery of Mn from mining wastes. The present investigation aims to report the bioleaching of Mn by Lysinibacillus sp. from mining waste residues in the context of mine waste remediation. Results confirmed that the native isolate had a high Mn biosolubilization potential with a solubilizing efficiency of 84% at the end of a 21day study under optimized conditions of pulp density 2% (< 150-μm particle size), pH 6.5, and temperature 30 °C. Fourier transform infrared spectroscopy (FTIR) studies followed by liquid chromatography mass spectrometry (LC-MS) analysis were used to ascertain the change in microbial protein conformation, configuration, and protein identification. The results revealed the expression of heat shock proteins (HSP) from the family HSP which is predominantly expressed in bacteria during stress conditions. This study represents the application of native bacterial strain in Mn biosolubilization. We foresee the utility of proteomics-based studies to provide a methodological framework to the underlying mechanism of metal solubilization, thereby facilitating the two-tier benefit of recovery of Mn from alternative sources as well as bioremediation of waste having high manganese content. Keywords Manganese . Lysinibacillus sp. . Biosolubilization . Recovery . LC-MS . HSP
Introduction The unprecedented demand for iron and steel has led to extensive global mining of high-grade manganese (Mn) ores. Around 15 million metric tons of manganese is used annually Responsible Editor: Diane Purchase * Alok Prasad Das [email protected] 1
Amity Institute of Biotechnology, Amity University, New Town, Kolkata 700135, India
2
Centre for Research in Nano Technology & Science (CRNTS), Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology Bombay, Mumbai, India
3
Institut de physique du globe de Paris, CNRS, Université de Paris, F-75005 Paris, France
4
Department of Life Science, Rama Devi Women’s University, Bhoinagar P.O, Bhubaneswar, Odisha 751002, India
for steel production at the global scale (Cui and Zhang 2008). High-grade ores are depleting fast, causing the necessity to utilize secondary sources of Mn like low-grade ores, mining scraps, electronic wastes, and mining effluents. Although sev
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