Integrated Metabolome and Transcriptome Analysis Reveal Complex Molecular Mechanisms Underlying Selenium Response of Alo
- PDF / 3,874,960 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 69 Downloads / 206 Views
RESEARCH ARTICLE
Integrated Metabolome and Transcriptome Analysis Reveal Complex Molecular Mechanisms Underlying Selenium Response of Aloe vera L. Yi Zou1 · Changyou Han2 · Fang Wang3 · Yanhua Tan2 · Saina Yang4 · Chuying Huang5 · Shengyou Xie6 · Xueqin Xiao1 Received: 7 April 2020 / Revised: 27 August 2020 / Accepted: 15 October 2020 © Korean Society of Plant Biologist 2020
Abstract Aloe vera L. is an excellent resource for medication. Selenium-enriched aloe can act as a functional food to human health. To understand the molecular mechanisms underlying selenium accumulation in Aloe vera L., we characterized the metabolic and transcriptome responses of aloe leaves under different Na2SeO4 levels (0, 200, and 400 mg/L) treatments. Aloe leaves spraying with exogenous selenium fertilizer showed a significant increase in total Se content compared with those under nontreatment control, and no distinct differences were observed between 200 and 400 mg/L Se treatment. Non-targeted metabolic profiling revealed that Se treatment triggered the accumulation of antioxidants, including amino acid and derivatives, phenols, flavonoids, terpene, as well as indole derivatives. Consistent with metabolic changes following Se treatment, the transcript level of genes involved in Se assimilation and Se-response showed dramatically increase, such as those encoding sulfate transporter, antioxidants, phytohormone signaling, transcription factors, and phenols metabolites, suggesting Se assimilation generally accompanied with antioxidant and pathogen defense. This study exhibited comprehensive insights on Se response in Aloe vera L., and provided us with targeted genes for genetic engineering, thereby improving the therapeutic value of aloe. Keywords Aloe vera L. · Se treatment · Metabolome · Transcriptome · Antioxidant response
Introduction Yi Zou, Changyou Han and Fang Wang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12374-020-09285-z) contains supplementary material, which is available to authorized users. * Xueqin Xiao [email protected] 1
The Central Laboratory, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
2
The Public Health Branch, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
3
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610000, China
4
The Finance Section, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
5
The Oncology Department, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
6
The General Affairs Section, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi 445000, China
Selenium (Se), as a structural component of selenoproteins, plays an essential role in immune, thyroid, and reproductive health for mammals, other animals, and some prokaryotes and algae (Rayman 2012; Zhang and Gladysh
Data Loading...
