Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching
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ORIGINAL ARTICLE
Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching in herbaceous peony Lei Liu1,2,3 · Yanqing Wu4 · Daqiu Zhao3 · Jun Tao2,3 Received: 9 August 2020 / Accepted: 14 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Herbaceous peony (Paeonia lactiflora Pall.) is a new high-end cut flower, but a large number of lateral branches often appear in some excellent cultivars, which is inconvenient for cut flower production. In the present study, we analyzed the effects of paclobutrazol (PBZ) on the lateral branches of P. lactiflora and adopted a next-generation sequencing approach to identify miRNAs and mRNAs that were differentially expressed involved in the PBZ response. Our results indicate that PBZ may inhibit the production of lateral branches on P. lactiflora. There were 827 differentially expressed genes (DEGs) and 104 differentially expressed miRNAs (DEMs). Integrative analysis revealed 29 miRNA–mRNA interactions related to PBZ stress. Our results provided a wealth of genetic information and data on metabolic pathways for revealing the regulatory mechanism of PBZ inhibition of the development of lateral branches in P. lactiflora and provided a new possibility for reducing lateral branch formation in the production of herbaceous peony cut flowers. Keywords Herbaceous peony · Paclobutrazol application · miRNA · Transcriptome sequencing · Integrated analyses
Introduction Herbaceous peony (Paeonia lactiflora Pall.) belongs to the Paeoniaceae family and is a traditional Chinese flower with a history of more than 3900 years of cultivation. It is considered “the flower phase” in China’s colorful gardens (Yuan and Yu 2011; Shen et al. 2012). In recent years, P. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02489-7) contains supplementary material, which is available to authorized users. * Jun Tao [email protected] 1
College of Horticulture, Xinyang College of Agriculture and Forestry, Xinyang 464000, China
2
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
3
Jiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
4
Institutes of Agricultural Science and Technology Development, Yangzhou University/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou 225009, China
lactiflora has been used in wedding bouquets and large floral arrangements and has very broad market prospects in Euro-American countries (Yu et al. 2011). The herbaceous peony cut flower industry standard not only requires a specific flower shape, flower color, flower aroma, petal texture, inflorescence stem and vase life but also requires no or few lateral branches (Lu and Yu 2009; Zhao et al. 2019). More lateral branches not only endlessly consume the nutrients in the plants
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