Interaction between serum albumins and sonochemically synthesized cadmium sulphide nanoparticles: a spectroscopic study
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RESEARCH PAPER
Interaction between serum albumins and sonochemically synthesized cadmium sulphide nanoparticles: a spectroscopic study Selvaraj Naveenraj • Abdullah M. Asiri Sambandam Anandan
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Received: 22 December 2012 / Accepted: 19 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Cadmium Sulphide nanoparticles approximately 5–10 nm in size range were synthesized by sonochemical technique, which follows acoustic cavitation phenomenon and generates nanoparticles with a smaller size range and higher surface area. The in vitro binding interaction of these sonochemically synthesized CdS nanoparticles with serum albumins (SA) were investigated using UV–Vis absorption, fluorescence and circular dichroism (CD) spectroscopic techniques since CdS nanoparticles has biological applications such as cellular labelling and deep-tissue imaging. UV–Vis absorption and fluorescence studies confirm that CdS nanoparticles bind with SA through ground state complex formation (static quenching mechanism). The results suggest that sonochemically synthesized CdS nanoparticles interact with HSA more than that of BSA and these nanoparticles can be easily transported and rapidly released to the targets by serum albumins. CD studies confirmed the conformational change of serum albumins on the interaction of CdS nanoparticles.
S. Naveenraj S. Anandan (&) Nanomaterials & Solar Energy Conversion Lab, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India e-mail: [email protected]; [email protected] A. M. Asiri The Center of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, Jidda 21413, P.O. Box 80203, Saudi Arabia
Keywords Sonochemical synthesis CdS nanoparticles Serum albumins Fluorescence quenching Circular dichroism Interaction studies
Introduction Owing to the unique quantum confinement size- and shape-dependent photoemissions, semiconductor chalcogenide nanoparticles are more intensely receiving tremendous fascinating applications in the fields of optics, electronics, catalysis, biology and medicine. In particular, semiconductor nanocrystals of cadmium sulphide (CdS) have attracted hefty attention due to their potential technological applications in solar cells, field effect transistors, photoconducting cells, sensors, nonlinear optics and heterogeneous photocatalysis (Moloto et al. 2007; Kumar et al. 2000; Prabhu and Khadar 2005). In addition, CdS nanoparticles found applications in biology and medicine for biomolecular recognition, cellular labelling and deep-tissue imaging due to the unique size-dependent physicochemical and optoelectronic properties (Lu et al. 2011; Li et al. 2006; Dooley et al. 2007). To the advantage of these biomedical applications, CdS nanoparticles have to be distributed to the targeted tissues through the circulatory system for the in vivo processes. The distribution depends on their binding nature with serum albumins, which is the most abundant and major soluble protein
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