Involvement of salicylic acid in cold priming-induced freezing tolerance in wheat plants
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ORIGINAL PAPER
Involvement of salicylic acid in cold priming-induced freezing tolerance in wheat plants Weiling Wang1,2 · Xiao Wang1 · Xinyu Zhang1 · Yao Wang1 · Zhongyang Huo2 · Mei Huang1 · Jian Cai1 · Qin Zhou1 · Dong Jiang1 Received: 17 March 2020 / Accepted: 28 September 2020 © Springer Nature B.V. 2020
Abstract Freezing is one of the most damaging abiotic stress factors. Cold priming has been shown to enhance freezing tolerance in wheat. However, the underlying mechanisms are unclear. Salicylic acid (SA) is an important signal molecule involved in plant responses to abiotic and biotic stresses. This study aims to investigate the role of SA in cold priming-induced freezing tolerance in wheat plants. The results showed that the expression of gene encoding phenylalanine ammonia-lyase (PAL, a key enzyme involved in SA biosynthesis) and the level of endogenous SA (both free and conjugated) were significantly upregulated following cold priming treatment. The role of SA in cold priming-induced freezing tolerance was further explored using the donor and inhibitor of endogenous SA. l-α-aminooxy-β-phenylpropionic acid (AOPP, a specific inhibitor of PAL) pretreatment reversed the increment of SA level induced by cold priming treatment, attenuated the positive effects of cold priming on antioxidant capacity (as indicated by the contents of MDA, ASA and GSH, and the activities of APX, GR, DHAR and MDHAR) and cold-responsive genes expression (such as AOX1a, IRI2 and HSP70) under the later freezing stress as well as the freezing tolerance (as indicated by the photochemical activity, electrolyte leakage and biomass). The application of exogenous SA could obviously counteract the negative effects of AOPP. Findings of the present study suggested that the roles of SA in cold priming-induced freezing tolerance in wheat might involve regulation of antioxidant capacity and coldresponsive gene expression. Keywords Antioxidant enzyme · Cold priming · Cold-responsive gene · Freezing tolerance · Salicylic acid · Wheat Abbreviations APX Ascorbate peroxidase AOPP l-α-aminooxy-β-phenylpropionic acid AOX Alternative oxidase ASA Ascorbate acid Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00671-8) contains supplementary material, which is available to authorized users. * Dong Jiang [email protected] 1
National Technique Innovation Center for Regional Wheat Production/Key Laboratory of Crop Physiology, Ecology and Production, MOA/National Engineering and Technology Center for Information Agriculture, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co‑Innovation Center for Modern Production Technology of Grain Crops/Agricultural College, Yangzhou University, No. 88 Daxue South Road, Yangzhou 225009, China
2
CBF/DREB C-repeat binding factor/dehydration responsive element binding protein COR Cold regulated DHAR Dehydroascorbic acid reductase Fv′/Fm′ The efficiency of exc
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