Iron nano modulated growth and biosynthesis of steviol glycosides in Stevia rebaudiana
- PDF / 1,949,085 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 79 Downloads / 146 Views
ORIGINAL ARTICLE
Iron nano modulated growth and biosynthesis of steviol glycosides in Stevia rebaudiana Mubarak Ali Khan1 · Amir Ali2 · Sher Mohammad2 · Huma Ali3 · Tariq Khan4 · Zia‑ur‑Rehman Mashwani5 · Asif Jan6 · Pervaiz Ahmad7 Received: 17 April 2020 / Accepted: 31 July 2020 © Springer Nature B.V. 2020
Abstract Application of nanomaterials is becoming the most effective strategy of elicitation to produce a desirable level of plant biomass with complex medicinal compounds. This study was, designed to check the influence of commercial iron nanoparticles (FeNPs) on physical growth characteristics, antioxidant status and production of steviol glycosides of in vitro grown Stevia rebaudiana. Results indicated that lower concentrations of FeNPs (45 µg/L) had a positive influence on morphological growth parameters. At a higher dose (90, and 135 µg/L) FeNPs in culture media were found detrimental to growth characteristics and development. Furthermore, the stress caused by FeNPs at 135 µg/L in cultures produced higher levels of total phenolic content (3.2 ± 0.042 mg/g dry weight: DW), total flavonoid content (1.6 ± 0.022 mg/g DW and antioxidant activity (73 ± 4.6%). In addition, plants grown in the presence of FeNPs at 90 µg/L resulted in higher enzymatic antioxidant activities (SOD = 3.2 ± 0.042 U/mg; POD = 2.1 ± 0.026 U/mg; CAT = 2.6 ± 0.034 U/mg and APx = 3.3 ± 0.043 U/ mg), respectively. Furthermore, exposure to a low dose of FeNPs (45 µg/L) exhibited the maximum amount of stevioside (stevioside: 4.2 ± 0.058 mg/g (DW) and rebaudioside A: 4.9 ± 0.068 mg/g DW) as compared to high doses. The current investigation confirms the effectiveness of FeNPs in growth media and offers a suitable prospect for commercially desirable production of S. rebaudiana biomass with higher sweet glycosides profiles in vitro. Key message Lower concentrations of Iron nano particles (FeNPs) had a positive influence on morphological growth parameters, production of antioxidant secondary metabolites and natural calorie free steviol glycosides in Stevia rebaudiana. Communicated by Mohammad Faisal. * Mubarak Ali Khan [email protected]; [email protected] 1
Department of Biotechnology, Faculty of Chemical and Life Sciences, Abdul Wali Khan University Mardan (AWKUM), Mardan 23390, Pakistan
2
Biotechnology Lab, Agricultural Research Institute (ARI), Tarnab Peshawar, Peshawar, Pakistan
3
Department of Biotechnology, Bacha Khan University, Charsadda, KP, Pakistan
4
Department of Biotechnology, University of Malakand, Chakdara, KP, Pakistan
5
Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan
6
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan
7
Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11362, Saudi Arabia
13
Vol.:(0123456789)
Plant Cell, Tissue and Organ Culture (PCTOC)
Keywords Stevia · Steviol glycosides · Cell culture · Iron nanoparticles · Elicitor · Micropropagation · Stevioside · Antioxidant · Secondary metabolites
Intr
Data Loading...
