Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabeti
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SHORT COMMUNICATION
Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats Paula Gimenez Milani1 • Silvano Piovan2 • Yago Carvalho Lima3 • Maria Rosa Trentin Zorzenon1 • Carlos Vinicius Dalto da Rosa4 • Giuliana Maria Ledesma Peixoto5 • Paulo Cezar de Freitas Mathias2 • Maria Raquel Marc¸al Natali6 • Silvio Claudio da Costa1 • Cecı´lia Edna Mareze-Costa5
Revised: 9 September 2020 / Accepted: 15 September 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract The study aims to analyse the treatment of whey protein enriched with Stevia rebaudiana fraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched with S. rebaudiana fraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of b cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched with S. rebaudiana fraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (* 0.13 ng/ mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (* 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These Paula Gimenez Milani and Silvano Piovan contributed equally to this work. & Paula Gimenez Milani [email protected] 1
Department of Biochemistry, State University of Maringa´, Maringa´, PR, Brazil
2
Department of Biotechnology, Genetics and Cell Biology, State University of Maringa´, Maringa´, PR, Brazil
3
Post-Graduate Program in Sciences (Human Physiology), State University of Sao Paulo, Sa˜o Paulo, SP, Brazil
4
University North of Parana´, UNOPAR, Bandeirantes, PR, Brazil
5
Department of Physiological Sciences, State University of Maringa´, Maringa´, PR, Brazil
6
Departament of Morphological Sciences, State University of Maringa, Maringa´, PR, Brazil
results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched with S. rebaudiana fraction can be used an adjunct/supplement in diabetic treatment. Keywords Insulin secretion Reduction of hyperglycemia Diabetes Supplementation Fraction of ethyl acetate
Introduction Type 1 diabetes mellitus (DM1) is characterized by apoptosis of pancreatic b cells and a critical reduction in insulin level with a consequent increase in blood glucose. Chronic hyperglycemia leads to increased protein glycation, causing damage to tissues and organs. When untreated, DM1 increases the risk of metabolic diseases, like as ketoacidosis, neuropathy, retinopathy, cardiovascular disorders, and nephropathy (Wada and Makino 2013). Several studies have shown that the use o
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