Isolation of a new Papiliotrema laurentii strain that displays capacity to achieve high lipid content from xylose
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ORIGINAL ARTICLE
Isolation of a new Papiliotrema laurentii strain that displays capacity to achieve high lipid content from xylose Nívea Moreira Vieira1 · Raquel Cristina Vieira dos Santos1 · Vanessa Kely de Castro Germano1 · Rafaela Zandonade Ventorim1 · Eduardo Luís Menezes de Almeida1 · Fernando Augusto da Silveira1 · José Ivo Ribeiro Júnior2 · Wendel Batista da Silveira1 Received: 11 March 2020 / Accepted: 31 July 2020 © King Abdulaziz City for Science and Technology 2020
Abstract In this work, we isolated and selected oleaginous yeasts from rock field soils from two National Parks in Brazil (Caparaó and Serra dos Órgãos) with the potential to accumulate oil from xylose, the main pentose sugar found in lignocellulosic biomass. From the 126 isolates, two were selected based on their lipid contents. They were taxonomically identified as Papiliotrema laurentii (UFV-1 and UFV-2). Of the two, P. laurentii UFV-1 was selected as the best lipid producer. Under unoptimized conditions, lipid production by P. laurentii UFV-1 was higher in glucose than in xylose. To improve its lipid production from xylose, we applied response surface methodology (RSM) with a face-centered central composite design (CCF). We evaluated the effects of agitation rate, initial cell biomass (OD600), carbon/nitrogen ratio (C/N ratio) and pH on lipid production. P. laurentii UFV-1 recorded the highest lipid content, 63.5% (w/w) of the cell dry mass, under the following conditions: C/N ratio = 100:1, pH value = 7.0, initial OD600 = 0.8 and agitation = 300 rpm. Under these optimized conditions, biomass, lipid titer and volumetric lipid productivity were 9.31 g/L, 5.90 g/L and 0.082 g/L.h, respectively. Additionally, we determined the fatty acid composition of P. laurentii UFV-1 as follows: C14:0 (0.5%), C16:0 (28.4–29.4%), C16:1 (0.2%), C18:0 (9.5–11%), C18:1 (58.6–60.5%), and C20:0 (0.7–0.8%). Based on this composition, the predicted properties of biodiesel showed that P. laurentii UFV-1 oil is suitable for use as feedstock in biodiesel production. Keywords Biodiesel · Lignocellulosic sugars · Oleaginous yeast · Response surface methodology · Screening
Introduction Concern about global warming has grown over recent years due to increases in the emission of greenhouse gases produced by the burning of fossil fuels. The report presented Nívea Moreira Vieira and Raquel Cristina Vieira dos Santos have contributed equally to this paper. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02373-4) contains supplementary material, which is available to authorized users. * Wendel Batista da Silveira [email protected] 1
Laboratory of Microbial Physiology, Department of Microbiology, Federal University of Viçosa, Av. P. H. Rolfs, s/n, Viçosa, MG 36570‑900, Brazil
Department of Statistic, Federal University of Viçosa, Viçosa, MG 36570‑900, Brazil
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in 2016 by Bloomberg New Energy Finance (BNEF), an organization that provides services to assist in the evaluation of investments in the
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