Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds
A new moderately halophilic bacterium strain SM. 200-5 was isolated from the solar saltern ponds with salinity 200 in Hangu Saltworks, Tianjin, China. The cells of the strain SM. 200-5 were rods and Gram-negative. They could grow in a salinity range of 30
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Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds Gaochao Xu, Yuangao Deng, Donghui Song and Liying Sui
Abstract A new moderately halophilic bacterium strain SM. 200-5 was isolated from the solar saltern ponds with salinity 200 in Hangu Saltworks, Tianjin, China. The cells of the strain SM. 200-5 were rods and Gram-negative. They could grow in a salinity range of 30–150 and initial pH 6–11, with optimum of salinity 100 and initial pH 7. 16S rDNA alignment showed that the strain SM. 200-5 had 93–95 % similarity with those sequences of genus Salimicrobium sp.. Biochemical characterization analysis via API 20E system indicated that biochemical characters of the strain SM. 200-5 were partially different from Salimicrobium sp. ISL-25, which was its closest member in the phylogenetic tree. Therefore, the strain SM. 200-5 proposed a new species of Salimicrobium. Keywords Halophilic bacterium 16S rDNA
Phylogenetic analysis Salimicrobium sp.
109.1 Introduction Halophilic bacteria are defined as those microorganisms inhabiting hypersaline environments such as salt lakes, salt mines, solar saltern ponds, and salted food [1, 2]. Moderately halophilic bacteria manifest optimal growth in salinity 30–150 [3]. In response of the hypersaline environment, halophilic bacteria usually have special physiological structures and produce valuable active compounds, such as poly-b-hydroxybutyrate (PHB), extracellular polysaccharides, ectoine, and thus possess the potential of biotechnological utilization [4–7]. Recent years many researches have focused on the taxonomy [8, 9] and biodiversity [10–13] of the G. Xu Y. Deng D. Song L. Sui (&) Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_109, Ó Springer-Verlag Berlin Heidelberg 2014
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halophilic bacteria, as well as their metabolites (e.g., bacteriorhodopsin, halocins) [14–17] and the ability to degrade pollutants (e.g., azo dye, hydrocarbon) [18, 19]. Halophilic bacteria play an important role in decomposing organic compounds in solar saltern ponds. High density of halophilic bacteria reduces the viscosity of the brine and enhances absorption of solar energy, and thus accelerates the evaporation rate of the brine and improves the quality and quantity of the salt yield [20]. A few studies have conducted in aspect of halobacterium in solar saltern ponds. Yoon et al. [21] isolated a halophilic bacterial strain MSS-155 from a marine solar saltern of the Yellow Sea in Korea; Montalvo-Rodríguez et al. [22] isolated three strains of halobacteria in the solar salterns in Cabo Rojo, Puerto Rico; Chen et al. isolated and identified moderately halophilic bacteria from solar saltern in Shan
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