Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles. Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers
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Maurizio Federico Editor
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools Edited by
Maurizio Federico National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
Editor Maurizio Federico Istituto Superiore di Sanità National AIDS Center Rome, Italy
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3751-6 ISBN 978-1-4939-3753-0 (eBook) DOI 10.1007/978-1-4939-3753-0 Library of Congress Control Number: 2016941747 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Lentiviral vectors (LVs) are very popular tools for stable cell engineering. The evolution of gene engineering of eukaryotic cells by LVs took great advantage from the establishment of innovative genetic and microbiologic technologies. In the present book, a consistent number of novel approaches based on the LV technology have been gathered, including new LV design and construction concepts, and improved strategies for cell targeting. In many instances, a time-restricted expression of the desired product in target cells in the absence of potentially dangerous transfer of genetic material is desirable, as in the case of immune adjuvant strategies. Exosomes, whose biogenesis is highly reminiscent of that of lentiviruses, can fill this gap. Notably, methods and reagents for exosome production and detection largely overlap those currently in use for lentiviral vectors. The inclusion in the same book of protocols for LV and exosome production can offer a wide range o
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