Levels of Extracellular Vesicles in Pulmonary and Peripheral Blood Correlate with Stages of Lung Cancer Patients
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ORIGINAL SCIENTIFIC REPORT WITH VIDEO
Levels of Extracellular Vesicles in Pulmonary and Peripheral Blood Correlate with Stages of Lung Cancer Patients Byeong Hyeon Choi1,2 • Yu Hua Quan1,2 • Jiyun Rho1,2 • Sunghoi Hong3 • Yong Park4 • Yeonho Choi5,6 • Ji -Ho Park7 • Hwan Seok Yong8 • Kook Nam Han2 • Young Ho Choi2 • Hyun Koo Kim1,2
Ó Socie´te´ Internationale de Chirurgie 2020
Abstract Background The extracellular vesicle (EV) concentration is known to be higher in cancer patients than in healthy individuals. Herein, we report that EV levels differ in the tumor-draining pulmonary vein blood and the peripheral blood of animal models and human subjects at different pathological stages of lung cancer. Methods Ten rabbits and 40 humans formed the study cohorts. Blood was collected from the peripheral vein of members of all groups. Pulmonary blood was collected intraoperatively from all groups except for the healthy human controls. Quantitative analysis of EV levels was performed using a nanoparticle tracking assay, a CD63 enzymelinked immunosorbent assay, and western blotting. Results The EV levels in the peripheral blood of animals and patients with lung cancer were higher than those in the peripheral blood of healthy controls (p \ 0.01 and p \ 0.001, respectively). Moreover, for both animals and patients with lung cancer, the EV levels in the pulmonary blood were significantly higher than those in the preoperative peripheral blood (p \ 0.01 and p \ 0.0001, respectively). In patients, the pathological stages of lung cancer showed a higher correlation with the pulmonary EV levels than the peripheral EV levels. Conclusions EV levels increased with increasing lung cancer grade, and this trend was more prominent in the pulmonary blood than in the peripheral blood.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00268-020-05630-y) contains supplementary material, which is available to authorized users. & Hyun Koo Kim [email protected]
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Department of Bio-Convergence Engineering, Korea University, Seoul, Republic of Korea
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Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea
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School of Biomedical Engineering, Korea University, Seoul, Republic of Korea
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Department of Thoracic and Cardiovascular Surgery, College of Medicine, Korea University Guro Hospital, Korea University, Seoul, Republic of Korea
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Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea
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School of Biosystems and Biomedical Sciences, College of Health Science, Korea University, Seoul, Republic of Korea
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Division of Hematology-Oncology, Department of Internal Medicine, College of Medicine, Korea University, Seoul, Republic of Korea
Department of Radiology, College of Medicine, Korea University Guro Hospital, Korea University, Seoul, Republic of Korea
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World J Surg
Introduction Lung cancer is a leading cause of cancer death worldwide [1]. Early diagnosis is lin
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