LhGST is an anthocyanin-related glutathione S-transferase gene in Asiatic hybrid lilies ( Lilium spp.)
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ORIGINAL ARTICLE
LhGST is an anthocyanin‑related glutathione S‑transferase gene in Asiatic hybrid lilies (Lilium spp.) Yuwei Cao1 · Leifeng Xu1 · Hua Xu1 · Panpan Yang1 · Guoren He1 · Yuchao Tang1 · Xianyu Qi1 · Meng Song1 · Jun Ming1 Received: 10 January 2020 / Accepted: 28 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message LhGST, an anthocyanin-related GST gene, was identified from Asiatic hybrid lilies. Expression and functional analyses demonstrated that LhGST might be involved in anthocyanin sequestration in lily tepals. Abstract Anthocyanins are responsible for the pink, red and purple pigmentation of flowers in Asiatic hybrid lilies, synthesized at the cytoplasmic surface of the endoplasmic reticulum (ER) and then transported to the vacuole. To date, the mechanism involved in the intracellular transport of anthocyanins in lilies has not been well elucidated. Here, full-length glutathione S-transferase gene (LhGST) was identified from lilies. Expression analysis revealed that LhGST was positively correlated with anthocyanin accumulation. Phylogenetic tree analysis showed that LhGST clustered with other anthocyaninrelated GSTs in the same phi clade. Moreover, functional complementation of an Arabidopsis tt19 mutant demonstrated that LhGST might be involved in anthocyanin accumulation in lily tepals. Additionally, according to phenotype analysis, LhGST was found to be correlated with the transport of anthocyanin in lilies by virus-induced gene silencing (VIGS) assay. In addition, cis-element analysis of the LhGST promoter showed the presence of ABA-, auxin-, MeJA-, gibberellin-, light-, and stress-responsive elements and an MYB recognition site (MRS, CCGTTG). Yeast one-hybrid and dual-luciferase report assays revealed that the promoter of LhGST was activated by LhMYB12-lat, which is a key R2R3-MYB transcription factor that regulates anthocyanin biosynthesis in lilies. In conclusion, our results revealed that LhGST plays a key role in anthocyanin transport and accumulation in the tepals of lilies. Keywords Lilium spp. · Glutathione S-transferases · Anthocyanins · Sequestration Abbreviations GST Glutathione S-transferase MATE Multidrug and toxic compound extrusion MRP Multidrug resistance-associated protein VIGS Virus-induced gene silencing TRV Tobacco rattle virus GFP Green fluorescent protein Communicated by Günther Hahne. Yuwei Cao and Leifeng Xu contributed equally to this manuscript. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02615-y) contains supplementary material, which is available to authorized users. * Jun Ming [email protected] 1
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Introduction Lilies (Lilium spp.) are among the most important ornamental horticultural plants because of their variety of flower colors, including red, pink, purple, orange, yellow, and white. Flower color is an important horticultural trait in lili
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