LINC01436 Inhibited miR-585-3p Expression and Upregulated MAPK1 Expression to Promote Gastric Cancer Progression
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ORIGINAL ARTICLE
LINC01436 Inhibited miR‑585‑3p Expression and Upregulated MAPK1 Expression to Promote Gastric Cancer Progression Yongpan Xu1 · Ming Dong2 · Jiehong Wang1 · Weihan Zhao1 · Min Jiao3 Received: 10 July 2019 / Accepted: 11 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background Gastric cancer (GC) is a prevalent type of digestion system malignancies. Dysregulation of long non-coding RNAs (lncRNAs) has been proven to be prognostic factors and biological regulators in human cancers. Aims The current study aimed to explore the role of long intergenic non-protein coding RNA 1436 (LINC01436) and its underlying mechanism in the progression of GC. Methods RT-qPCR was conducted to measure RNA expression. Western blot was used for exploration of protein level. CCK-8, caspase-3 activity, and transwell assays were applied to evaluate the proliferative, apoptotic, and migratory abilities of GC cells, respectively. Mechanical experiments were used to probe the molecular interplay between genes. Results High LINC01436 level suggested low overall survival in GC patients, and LINC01436 was highly expressed in GC tissues and cells. Besides, LINC01436 knockdown hampered cell proliferation and migration, while facilitated cell apoptosis. Mechanistically, LINC01436 upregulated mitogen-activated protein kinase 1 (MAPK1) expression by competitively binding with miR-585-3p and inhibiting miR-585-3p expression. Furthermore, LINC01436 negatively regulated miR-585-3p expression by enhancing the zeste 2 polycomb repressive complex 2 subunit (EZH2)-induced trimethylation of histone H3 at lysine 27 (H3K27me3) on miR-585-3p promoter. Final rescue assays revealed that overexpression of MAPK1 could rescue the suppressive influence of LINC01436 depletion on GC progression. Conclusions LINC01436 epigenetically silences miR-585-3p and acts as miR-585-3p to upregulate MAPK1 expression and promote GC progression. Keywords LINC01436 · miR-585-3p · MAPK1 · EZH2 · H3K27me · GC
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10620-020-06487-w) contains supplementary material, which is available to authorized users. * Min Jiao [email protected] 1
Department of Gastroenterology, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, Shaanxi, China
2
Department of Surgical Oncology, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, Shaanxi, China
3
Department of Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Xi’an 710061, Shaanxi, China
Gastric cancer (GC) is a prevalent cancer type among the malignancies of digestion system with increasing occurrence rate [1]. Unfortunately, the treatment tools for GC patients are not efficient enough and GC patients at advanced stages are usually faced with malignant proliferation and metastasis [2, 3]. Therefore, identification of new molecular target in GC is necessitated
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