LncRNA LEF1-AS1 promotes osteogenic differentiation of dental pulp stem cells via sponging miR-24-3p
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LncRNA LEF1‑AS1 promotes osteogenic differentiation of dental pulp stem cells via sponging miR‑24‑3p Yuexia Wu1 · Keqian Lian2 · Cong Sun3 Received: 31 May 2020 / Accepted: 1 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The role of lncRNA LEF1-AS1 in the regulation of osteogenic differentiation of dental pulp stem cells (DPSCs) is still obscure. Here, we demonstrated that LncRNA LEF1-AS1 expression was associated with osteogenic differentiation of DPSCs and overexpression of LEF1-AS1 promoted osteogenic differentiation. Moreover, lncRNA LEF1-AS1 and miR24-3p could directly regulate each other and LEF1-AS1 acted as sponge partner of miR-24-3p. Furthermore, LEF1-AS1 and miR-24-3p synergized to regulate osteogenic differentiation of DPSCs. Finally, we verified TGFBR1 was the direct target of miR-24-3p in osteogenic differentiation of DPSCs and miR-24-3p/LEF1-AS1 sponged to regulate TGFBR1 expression. Our study revealed a novel mechanism about how did lncRNA LEF1-AS1 execute function in osteogenesis of DPSCs and thus might serve as potential therapeutic target for the bone regeneration in the dental pulp. Keywords LEF1-AS1 · miR-24-3p · TGFBR1 · DPSC · LncRNA · Osteogenesis
Introduction The pulp tissue is located in the pulp cavity inside the tooth, which is the only soft tissue in the tooth [1, 2]. Fibroblasts isolated from Pulp tissue are called dental pulp stem cells (DPSCs) [3]. Dental pulp stem cells have multidirectional differentiation potential [4]. In addition to cells capable of forming mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelium, liver, nerve and other cell lines after induction of different cytokines [5–7]. More than 14 million root canal operations are performed each year to treat bacterial pulpitis, characterized by inflamed pulp and periapical tissues [8]. The occurrence of pulpitis will lead to the loss of regeneration ability of dental pulp cells, making the complete healing of pulpitis Yuexia Wu and Keqian Lian are contributed equally. * Cong Sun [email protected] 1
Stomatological Hospital, Southern Medical University, Guangzhou 510280, Guangdong, China
2
The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong, China
3
The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shanxi, China
difficult [9]. Thus, the discovery of effective treatment targets for pulpitis with little side effects is quite essential. To date, knowledge about the regulation of DPSCs differentiation still remained vague. Long non-coding RNAs (lncRNAs) are RNAs with a length of more than 200 nucleotides and are not translated into proteins [10–12]. Their functional disorders and specific roles in various diseases, especially cancers, have attracted increasing attention [13, 14]. Alterations or mutations in the lncRNAs have been reported to modulate multiple physiological processes [14]. LEF1-AS1 (LEF1 antisense RNA 1), encoded in the lymphoid enhancer‐binding
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