Effects of resveratrol on the proliferation and osteogenic differentiation of deciduous dental pulp stem cells from neur
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ORIGINAL ARTICLE
Effects of resveratrol on the proliferation and osteogenic differentiation of deciduous dental pulp stem cells from neurofibromatosis type 1 patient Claudinéia Pereira Maranduba 1 & Gustavo Torres Souza 1 & Antônio Márcio Resende do Carmo 2 & José Marcelo Sallabert de Campos 3 & Nádia Rezende Barbosa Raposo 4 & Marcelo de Olivera Santos 5 & Carlos Magno da Costa Maranduba 3 & Fernando de Sá Silva 5 Received: 22 June 2020 / Accepted: 6 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Purpose We aimed at verifying whether resveratrol can decrease cell proliferation and change osteogenic differentiation of cells obtained from patients with type 1 neurofibromatosis (NF1). Methods Deciduous dental pulp derived stem cells were isolated from NF1 patient and healthy volunteer. These cells were subjected to increasing concentrations of resveratrol and evaluated for proliferation and mineralization of osteogenic differentiation. Results The results showed that resveratrol reduced the difference in proliferation between CNT and NF1 cells in a dosedependent manner and this property was more prominent in affected cells than in healthy cells. Resveratrol showed no statistically significant changes in mineralization in osteogenic differentiation of NF1 cells, at low doses tested. Conclusions In conclusion, in a dose-dependent manner, resveratrol displays interesting properties that could be applied in a possible treatment aimed at decreasing cellular proliferation in neurofibromatosis. Furthermore, it is selective concerning healthy cells and not affecting cell differentiation. Further research to cell selectivity, differentiation to other tissue types, and cell cytotoxicity are needed. Keywords Stem cells . Proliferation . Resveratrol . Osteogenic differentiation . Deciduous dental pulp stem cells . Neurofibromatosis
Introduction Carlos Magno da Costa Maranduba and Fernando de Sá Silva share last authorship * Carlos Magno da Costa Maranduba [email protected] 1
Laboratory of Human Genetics and Cell Therapy, Biology Department, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil
2
Department of Endodontology, Dentistry School, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil
3
Laboratory of Genetics, Biology Department, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil
4
Center of Research and Innovation in Health Sciences (NUPICS), Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil
5
Basic Life Sciences Department, Federal University of Juiz de Fora, Governador Valadares 35020-670, Brazil
Neurofibromatosis type 1 (NF1) is the most common of the inherited diseases called neurofibromatoses. It is caused by a mutation in the neurofibromin gene (NF) in which patients have a high probability of developing tumors derived from cells of the nervous system. The tumors are named neurofibromas, which develop in peripheral nerve sheath const
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