LncRNA PCAT6 Regulated by YY1 Accelerates the Progression of Glioblastoma via miR-513/IGF2BP1
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ORIGINAL PAPER
LncRNA PCAT6 Regulated by YY1 Accelerates the Progression of Glioblastoma via miR‑513/IGF2BP1 Peng Liu1 · Peng Zhao1 · Bing Li1 · Dianxiang Xu2 · Kun Wang1 Received: 3 July 2020 / Revised: 20 September 2020 / Accepted: 23 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Glioblastoma is one of the most frequent and aggressive primary tumor of glial brain tumors. Long non-coding RNA Prostate cancer-associated ncRNA transcript 6 (PCAT6) has been identified to influence the progression of many cancers, but its expression and functions in glioblastoma remain unclear. In this study, we intended to investigate the expression, functions and the corresponding mechanisms of PCAT6 in glioblastoma. We observed that PCAT6 expression was upregulated in glioblastoma tissues and cell lines and its high expression was due to the transcriptional activation by Yin Yang 1. miR-513 was a target of PCAT6 and Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) was a target of miR-513. Hence, PCAT6 upregulated IGF2BP1 expression via miR-513 in a competing endogenous RNAs manner. PCAT6 and IGF2BP1 functioned as oncogenes while miR-513 acted as a tumor suppressor gene in glioblastoma. PCAT6 and miR-513 modulated the proliferation and survival of glioblastoma cells via AKT signaling by mediating IGF2BP1. IGF2BP1 raised the expression of PCAT6 by increasing its stability. In conclusion, our results indicate that PCAT6/miR-513/IGF2BP1 positive feedback loop plays a crucial role in facilitating glioblastoma progression. Keywords Glioblastoma · Long non-coding RNA · PCAT6 · miR-513 · Yin yang 1 · IGF2BP1
Introduction Glioma is one of the most common primary brain tumor [1] and glioblastoma (GBM) represents the most aggressive glioma [2]. The prognosis of GBM patients is pretty poor due to the high degree of malignancy [3], although tremendous advances in treatment have been made in recent years. Therefore, it is crucial to better understand the molecular mechanisms underlying GBM development and find effective targets for the treatment of GBM. Long non-coding RNAs (LncRNAs) are a class of noncoding RNAs with lengths larger than 200 nucleotides. Abnormally expressed lncRNAs have been reported in various diseases and found to play important roles in the disease
course [4–7]. According to many studies, some lncRNAs are also involved in the tumorigenesis of glioblastoma [8–10]. Prostate cancer-associated ncRNA transcript 6 (PCAT6) has been recognized to be upregulated in a variety of human cancers including hepatocellular carcinoma, colorectal cancer, cervical cancer, colon cancer, non-small-cell lung carcinoma and proved to function as an oncogene in these cancers [11–15]. However, the expression and functions of PCAT6 in GBM remain unclear. Our present study attempted to probe into the expression and functions of PCAT6 in GBM and further investigate the underlying mechanisms.
Materials and Methods Tissue Samples
* Kun Wang [email protected] 1
Department of Neurosurger
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