miR-23a-3p regulated by LncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via
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miR-23a-3p regulated by LncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5 Zhen Yang 1,2 & Zhijing Ren 1,2 & Rongfeng She 2 & Jun Ao 3 & Qingde Wa 3 & Zeyu Sun 2 & Bo Li 2 & Xiaobin Tian 4 Received: 19 January 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Cartilage generation and degradation are controlled by miRNAs. Our previous study showed miR-23a-3p was downregulated during chondrogenic differentiation in chondrogenic human adipose-derived mesenchymal stem cells (hADSCs). In the present study, we explored the function of miR-23a-3p in chondrogenesis differentiation. The role of miR-23a-3p in chondrogenic differentiation potential of hADSCs was assessed by Alcian blue staining, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. We show that miR-23a-3p suppressed the chondrogenic differentiation of hADSCs. LncRNA SNHG5 interacted with miR-23a-3p, and suppression or overexpression of SNHG5 correlates with inhibition and promotion of hADSC chondrogenic differentiation, respectively. We have determined that SNHG5 can sponge miR-23a-3p to regulate the expression of SOX6/SOX5, transcription factors that play essential roles in chondrocyte differentiation. Furthermore, the overexpression of SNHG5 activates the JNK/MAPK/ERK pathway. In conclusion, miR-23a-3p regulated by lncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5. Keywords miR-23a-3p . SNHG5 . Chondrogenic differentiation . hADSC . SOX6/SOX5
Introduction Osteoarthritis (OA) is a progressive joint disease involving mainly degeneration of articular cartilage and inflammation of the synovium, causing joint pain and significantly reducing motor functions (Andarwulan et al. 2010). The prevalence rate of OA in people over 60 years old is higher than that of lumbago, neck and shoulder pain, inflammatory arthritis, osteoporosis, and spinal deformity, and OA has been one of the * Bo Li [email protected] * Xiaobin Tian [email protected] 1
Medical College of Guizhou University, Guiyang 550025, Guizhou, China
2
Department of Orthopedics, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou, China
3
Department of Orthopedics, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou, China
4
Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550001, Guizhou, China
main diseases leading to the decline of activity dysfunction and quality of life in the middle-aged and older people (Pereira et al. 2011). Autogenous chondrocyte implantation is widely used in cartilage defect repair, and its clinical effect has been confirmed (Komarek et al. 2010; Niethammer et al. 2016). However, the sources of autologous chondrocytes are limited, and the materials are inconvenient (arthroscopy or surgical materials are required); the ability of traditional culture to pass through and proliferate is lim
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