Magnetically controlled colorimetric aptasensor for chlorpyrifos based on copper-based metal-organic framework nanoparti
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ORIGINAL PAPER
Magnetically controlled colorimetric aptasensor for chlorpyrifos based on copper-based metal-organic framework nanoparticles with peroxidase mimetic property Qingju Liu 1,2
&
Zhaoying He 1,2 & Hui Wang 1,2 & Xiaoyuan Feng 1,2 & Ping Han 1,2
Received: 7 January 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The fabrication of a magnetically controlled colorimetric aptasensor for chlorpyrifos is reported. The aptasensor was fabricated by the attachment of the colorimetric labels onto the magnetic carrier due to the hybridization reaction between the complementary DNA and aptamer. Chlorpyrifos detection was realized by monitoring the color changes of the TMB/H2O2 solution before and after incubation of the aptasensor with chlorpyrifos via exposure to external magnetic force. The color change was monitored at 650 nm by UV-Vis spectrophotometer. Under the optimal conditions, this magnetically controlled Cu-MOF-based aptasensor showed a detection limit of 4.4 ng/mL with a linear range of 0–1250 ng/mL. The colorimetric aptasensor displayed high selectivity for chlorpyrifos toward other interfering pesticides. The aptasensor was successfully applied for the spiked test of chlorpyrifos in fruits and vegetable samples with good recovery, which were in agreement with data obtained by GC-MS analysis. This magnetically controlled Cu-MOF-based sensing strategy not only leads to development of efficient and facile phase separation, but also expands the MOF’s target scope from H2O2 or glucose to pesticides. Keywords Metal-organic framework . Peroxidase mimetic . Chromogenic reaction . Chlorpyrifos
Introduction As a substitute for conventional pesticides with high toxicity, chlorpyrifos (CPF) can release and accumulate in water and food chain which will pose a risk to health due to its potential toxicity such as lung toxicity [1], intracerebroventricularly and systemically delivered inhibitor of brain CYP2B [2], cholinergic neurotoxicity [3], and neurodevelopment [4]. Therefore, it is urgent to develop CPF analytical sensors for routine and effective monitoring. Different analytical methods such as ELISA for pesticide residues have been developed by using natural enzymes [5]. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04499-x) contains supplementary material, which is available to authorized users. * Ping Han [email protected] 1
Beijing Research Center for Agriculture Standards and Testing, Beijing 100097, China
2
Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture, Beijing 100097, China
Horseradish peroxidase (HRP) is the most widely used enzyme, and the substrate of HRP is peroxide, such as hydrogen peroxide. Because of high efficiency, no toxicity, no carcinogenicity, and good stability, 3,3′, 5,5′-tetramethylbenzidine (TMB) is a common substrate of HRP. HRP catalyzes peroxide to release oxygen, and colorless TMB is oxidized into blue product, and the absorbance of bl
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