Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs
- PDF / 711,554 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 39 Downloads / 163 Views
Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs Thomas W. Fallows†,1, 2 Thomas P. Coxon†,1, 2 Julie E. Gough2 and Simon J. Webb1 1 School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK 2 School of Materials, University of Manchester, MSS Tower, Manchester, M13 9PL, UK † These authors contributed equally to this work. ABSTRACT Providing magnetite nanoparticles with saccharide coatings has been found to significantly increase the interactions of the nanoparticles with cells. Glucose (Glc) or Nacetylglucosamine (GlcNAc) coated magnetic nanoparticles (MNPs) were used to magnetically label 3T3 fibroblast cells, and the response of the labelled cells to external magnetic fields was studied. It was found that cells incubated with Glc- or GlcNAc-coated nanoparticles were much more likely to move towards an external magnet than those incubated with uncoated nanoparticles. Furthermore, cells in suspension moved much faster than those in contact with the surface of polystyrene well plates, with stronger magnets increasing the speed of movement. Cells that were adhering to the floor of the cell culture well and did not move in the x-y plane could still be rotated about the z-axis by moving the external magnet around the cell. INTRODUCTION Magnetite nanoparticles are used in a wide variety of biomedical applications,1, 2 so understanding cell-MNP interactions is of keen interest. Externally added MNPs are often taken up by cells in vitro and in vivo, resulting in magnetic labelling of those cells. This labelling allows for the manipulation of the cells via magnetophoresis,3 whereby the cells move when placed in an external magnetic field. There are many biomedical applications for magnetophoresis, for example, external control over the location of nanoparticles in vivo for therapeutic and diagnostic purposes,4 the removal of specific cell lines suspended in blood5 and separation of cell lines for screening and research.6, 7 In order to improve MNP-mediated magnetophoresis of cells, the nanoparticles should be coated with a biocompatible agent to improve adhesion to and uptake by the target cells. Common reagents for coating magnetic nanoparticles include dextran,8 3-aminopropyltriethoxysilane (APTES)9 and polyethylene glycol (PEG),10 but none of these molecules are able to target particular cell types through specific interactions. In contrast, saccharide-coated nanoparticles are able to exploit cells’ natural recognition pathways by binding to cell-surface lectins, taking advantage of the strong and specific binding produced by the multiple individual binding events,11-13 known as the cluster glycoside effect.14 Since catechols15 and resorcinols16 are known to bind to the surface of Fe3O4 nanoparticles, these functional groups were chosen to anchor the cell targeting saccharides to the MNPs. Saccharide-terminated coating molecules have previously been synthesized by reacting a catechol or resorcinol hydrazide with a reduci
Data Loading...
