Mammalian Synthetic Promoters
This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecula
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David Gould Editor
Mammalian Synthetic Promoters
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mammalian Synthetic Promoters Edited by
David Gould Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK
Editor David Gould Department of Biochemical Pharmacology William Harvey Research Institute Queen Mary University of London London, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7221-0 ISBN 978-1-4939-7223-4 (eBook) DOI 10.1007/978-1-4939-7223-4 Library of Congress Control Number: 2017946166 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface It is nearly 40 years since the TATA box was first identified. Since this time there has been a revolution in genetics research and an increase in the ways that promoters are utilized. Current applications of promoters include their use in biotechnology for expression of recombinant proteins; as switches or logic gates in synthetic biology; for spatiotemporal expression in gene targeting; disease, tissue-specific, and constitutive expression in gene therapy; and monitoring transcription factor activity and numerous applications for expression of genes of interest in basic biological research. In this volume a number of methods are outlined that will aid in the selection of promoter sequences and vector components, methods for the assembly and testing of synthetic p
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