Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels
- PDF / 630,867 Bytes
- 12 Pages / 547.087 x 737.008 pts Page_size
- 38 Downloads / 191 Views
Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels Emil Stefańczyk & Jarosław Plich & Marta Janiszewska & Paulina Smyda-Dajmund Sylwester Sobkowiak & Jadwiga Śliwka
&
Received: 8 June 2020 / Accepted: 28 August 2020 / Published online: 5 September 2020 # The Author(s) 2020
Abstract Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the late blight resistance gene Rpi-rzc1 from Solanum ruiz-ceballosii to the tetraploid level of cultivated potato and to pyramid it with the Rpi-phu1 gene. We obtained two diploid and, through 4x-2x cross, a tetraploid potato population segregating for the Rpi-rzc1 presence, as well as one diploid and one tetraploid population where both genes were introgressed. In total, 754 progeny clones were tested for resistance to late blight in detached leaflet assays. Pathogen isolates avirulent on plants with both genes and virulent on plants with the Rpi-phu1 were used. The selection was assisted by two PCR markers flanking the Rpi-rzc1 gene and a newly designed, highly specific intragenic marker indicating the Rpi-phu1 gene presence. We obtained 26 diploid and 49 tetraploid potato clones with pyramid of both genes that should enhance Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01169-x) contains supplementary material, which is available to authorized users. E. Stefańczyk : J. Plich : M. Janiszewska : P. Smyda-Dajmund : S. Sobkowiak : J. Śliwka (*) Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland e-mail: [email protected]
the durability and spectrum of their late blight resistance and that can be exploited in potato breeding. The specificity of the marker for the Rpi-phu1 gene and the precision of the Rpi-rzc1 mapping were improved in this work. Keywords 13_A2 . Gene stacking . Interploid cross . Phytophthora infestans . R gene . Solanum tuberosum
Introduction The cultivated potato, Solanum tuberosum L., and its wild relatives originate from South and Central America. Over 200 species of Solanum section Petota, to which S. tuberosum belongs, include species of various ploidy levels (diploids, tetraploids, hexaploids). Their taxonomy is affected by introgressions, interspecific hybridisations, sexual compatibility and phenotypic plasticity. They show diversity in plant height, tuber, leaf and flower colours and shapes (Ovchinnikova et al. 2011; Spooner et al. 2004). Wild potato relatives are adapted to plethora of environments that involve different precipitation regimes, day lengths, soils or latitudes and altitudes (Machida-Hirano 2
Data Loading...
