Metabolic changes in follicular fluids of patients treated with recombinant versus urinary human chorionic gonadotropin
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GYNECOLOGIC ENDOCRINOLOGY AND REPRODUCTIVE MEDICINE
Metabolic changes in follicular fluids of patients treated with recombinant versus urinary human chorionic gonadotropin for triggering ovulation in assisted reproductive technologies: a metabolomics pilot study Maria Antonietta Castiglione Morelli1 · Assunta Iuliano2 · Sergio Crescenzo Antonio Schettini2 · Donatina Petruzzi2 · Angela Ferri2 · Paola Colucci2 · Licia Viggiani1 · Angela Ostuni1 Received: 20 December 2019 / Accepted: 19 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Introduction The main goal of this retrospective cohort study is the assessment of the effects of administration of recombinant-hCG (r-hCG) versus urinary-hCG (u-hCG) on follicular fluid (FF) composition of women who underwent in vitro fertilization (IVF) treatments. Materials and methods We selected 70 patients with infertility attributable to tubal diseases, unexplained infertility, and male factor. Metabolomics analysis of their FFs was performed by 1H nuclear magnetic resonance (1H NMR) spectroscopy in combination with multivariate analysis to interpret the spectral data. Univariate statistical analysis was applied to investigate the possible correlations between clinical parameters and between clinical parameters and metabolites identified by NMR. Results According to the type of hCG used, significant differences were detected in FFs of women with male factor and unexplained infertility, both in qualitative and quantitative terms, for some metabolites as cholesterol, citrate, creatine, β-hydroxybutyrate, glycerol, lipids, amino acids (Glu, Gln, His, Val, Lys) and glucose. No significant difference was observed in women with tubal diseases. Besides, the number of MII oocytes in the u-hCG-treated groups correlates positively with glutamate in tubal disease and with glycerol in unexplained infertility. In the r-hCG-treated groups, the number of MII oocytes correlates positively with lipid in tubal disease, positively with citrate and negatively with glucose in male infertility. Conclusions Metabolite composition of FF changes according to different type of hCG treatment and this can be related to oocyte development and subsequent outcome. According to the data of this study, different types of hCG should be used in relation to the diagnosis of infertility to obtain better results in inducing oocyte maturation in women undergoing IVF. Keywords Recombinant hCG · Urinary hCG · Assisted reproduction technology · NMR · Metabolomics · Biomarkers · Follicular fluid
The authors consider that the first two authors should be regarded as joint first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00404-020-05609-z) contains supplementary material, which is available to authorized users. * Angela Ostuni [email protected] 1
Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100 Potenza, Italy
Center for Reproductive Medicine of “San Carlo” Hospital, via Potito Pe
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