Methanolic extracts of a selected Egyptian Vicia faba cultivar mitigate the oxidative/inflammatory burden and afford neu
- PDF / 3,065,093 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 18 Downloads / 167 Views
Inflammopharmacology
ORIGINAL ARTICLE
Methanolic extracts of a selected Egyptian Vicia faba cultivar mitigate the oxidative/inflammatory burden and afford neuroprotection in a mouse model of Parkinson’s disease Essam Abdel‑Sattar1,6 · Engy A. Mahrous1,6 · Mareena M. Thabet2 · Dina M. Yousry Elnaggar2 · Amal M. Youssef3 · Reda Elhawary4 · Sawsan A. Zaitone5,6 · Celia Rodríguez‑Pérez7,8 · Antonio Segura‑Carretero7,8 · Reham Hassan Mekky2,7 Received: 20 May 2020 / Accepted: 3 October 2020 © Springer Nature Switzerland AG 2020
Abstract Vicia faba L. is a legume from the family Fabaceae. Ancient Egyptians consumed fava beans thousands of years ago and they are still one of the most popular foods in Egypt. The current study examined the anti-Parkinson effect of 80% methanolic extracts of seeds or sprouts of the fava ‘Sakha 3 ‘cultivar which has been selected based on the total phenol content among three cultivars tested. In addition, the extracts were characterized by reversed-phase high-performance liquid chromatography coupled with diode array detection and quadrupole-time-of-flight-mass spectrometry (RP-HPLC–DAD-QTOF-MS). Three doses (200, 400, and 600 mg/kg) of 80% methanol extracts of seeds or sprouts of the Sakha 3 cultivar were evaluated in rotenone–Parkinsonian mice from behavioral, biochemical, and histopathological aspects. The extract of fava sprouts (600 mg/ kg dose) showed the most beneficial effect. It improved motor activity, enhanced striatal dopamine level, and decreased the striatal malondialdehyde, as well as the expression of the inflammatory markers, compared with the rotenone control group and groups receiving lower therapeutic doses of the extracts or l-Dopa. In addition, these findings were supported by a histopathological investigation which indicated that mice treated with the 600-mg/kg dose of the sprout extract showed a low number of degenerated neurons. The application of RP-HPLC–DAD-QTOF-MS and mass/mass spectroscopy enabled the metabolic profiling of the sprouts and seeds of the ‘Sakha 3′ cultivar. It is obvious that germination increased the amounts of phenolic acids, saponins, and aromatic amino acids, together with a dramatic increase in flavonoids. In conclusion, the 80% methanolic extract of sprouts of the fava “Sakha 3” cultivar may be a promising candidate for treating Parkinsonism if appropriate safety data are available. Keywords Antioxidant · Anti-inflammatory · Neuroprotection · Parkinsonian mice · Vicia faba l. · LC–MS · MS
Introduction Parkinson’s disease (PD) is a nervous system disorder that is described as neuroinflammation and progressive damage in the substantia nigra dopamine neurons leading to Essam Abdel-Sattar and Engy A. Mahrous have equal contribution and are considered as first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10787-020-00768-6) contains supplementary material, which is available to authorized users. * Essam Abdel‑Sattar [email protected] Extended author information av
Data Loading...
