Microbial colonization on the leaf surfaces of different genotypes of Napier grass
- PDF / 2,346,075 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 198 Views
ORIGINAL PAPER
Microbial colonization on the leaf surfaces of different genotypes of Napier grass Guojian Tang1 · Liuxing Xu1 · Xiang Yin1 · Yaqin Hu1 · Jing Tian1 · Jianguo Zhang1 Received: 24 May 2020 / Revised: 13 August 2020 / Accepted: 2 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract To address correlations between population sizes of microbes on the leaf surfaces and leaf morphological and physicochemical properties, various leaf morphological and physicochemical features as possible predictors of microbial population sizes on the leaf surfaces of four Napier grass cultivars were assessed. Results indicated microbes except for lactic acid bacteria (LAB) preferred to colonize the leaf surfaces bearing trichomes, and their population sizes were significantly correlated with trichomes, especially for yeasts. The population sizes of microbes were positively correlated with soluble sugar content (p 73%) were more colonized, and small microbial population was associated with higher wax content (> 10.66 mg g−1 dry matter). In conclusion, leaf chemical attributes have a higher contribution than morphological structure properties in determining population sizes of microbes on the leaf surfaces. The exuded soluble sugar and protein promote the development of microbial populations. For different genotypes of leaf–microbe system, the relationship between microbial abundance on their leaf surfaces and leaf morphological structure or physicochemical properties may be predicted by the MRT. Population sizes of microbes are primarily influenced by soluble sugar content under the water-rich conditions. Keywords Plant genotype · Epiphytic microbes · Leaf surface traits · Leaf–microbe interaction
Introduction Leaf surface of plants, also called the phyllosphere (Vorholt 2012), locating at the interface between a plant and its aerial environment, is covered by the plant cuticle (Jeffree 2006). Leaf epidermal cuticles observably protect terrestrial plant from water loss and leaching of plant metabolites from the leaf apoplast to the leaf surfaces (Jeffree 2006). Bacteria are the most dominant groups of microorganisms on the leaf surfaces, which reach an astonishing population of averagely Communicated by Erko stackebrandt. * Guojian Tang [email protected] 1
South Pratacultural Center, South China Agricultural University/Guangdong Engineering Research Center of Grassland Science, Guangzhou 510642, China
4–5 log10 cfu m m−2 (where cfu means colony forming units), or up to 8 log10 cfu g −1 fresh leaves (Remus-Emsermann et al. 2014). The population sizes of microorganisms living on the leaf surfaces vary both among and within cultivars. Phyllosphere microbes are significantly affected by leaf age, position and season or growing conditions (Jacques 1996). The environmental conditions influence the processes of microbial immigration, emigration, growth and death. The development of microbial communities must be largely related to the phenotypic characteristics of plants, which
Data Loading...
