Micropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest
- PDF / 1,432,192 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 212 Views
ORIGINAL ARTICLE
Micropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest Jean Santos Machado1 · Juliana Degenhardt2 · Fabiano Rodrigo Maia1 · Marguerite Quoirin1 Received: 26 April 2019 / Accepted: 22 January 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Our study shows for the first time a complete guabiroba micropropagation system with acclimatized plants. BA was the best plant growth regulator for multiplication and IBA for rooting. Abstract Campomanesia xanthocarpa O. Berg (Myrtaceae), popularly known as guabiroba, is a woody species native to Brazil, important for its potential as a medicinal plant and fruit tree. For the first time, this study shows a complete system of micropropagation for this species. For this, nodal segments with two axillary buds each were used as starting material. Plant Preservative Mixture™ (0.1%) added to culture media was efficient in controlling contamination throughout the culture process. Two formulations of culture medium were compared for in vitro establishment. 2-Isopentenyladenine, 6-benzyladenine, kinetin and zeatin were tested during the multiplication step. Woody plant medium (WPM) was appropriate for all culture steps and, during the multiplication process, 2.2 µM BA induced the best numbers of new shoots per explant during three subcultures (3.0–3.5 per month). For rooting of microcuttings, a rate of 53% was reached in the WPM supplemented with 4.9 µM indol-3-butyric acid. A plastic box containing a mixture of commercial substrate and vermiculite (1:1 v/v) was used for plantlet acclimatization, allowing 52% of survival after two months. In conclusion, a complete micropropagation protocol was developed providing healthy plants. Further studies are needed to improve multiplication and survival rates. Keywords Cytokinin · Guabiroba · Plant preservative mixture · Woody plant medium
Introduction Campomanesia xanthocarpa O. Berg (Myrtaceae) is a woody species native to the Savanna and Atlantic Forest of Brazil, popularly known as guabiroba or gabiroba (Sobral et al. 2016). This species has medicinal potential and is used in folk medicine for the treatment of gastric ulcers (Markman et al. 2004) and to control blood glycemia (Biavatti et al. 2004; Vinagre et al. 2010; Viecili et al. 2014) and cholesterol levels (Klafke et al. 2010). Its medicinal proprieties are due to the phenolic compounds, such as flavones and chalcones, present in the leaves, fruits, and bark (Schmeda-Hirschmann Communicated by Merkle. * Jean Santos Machado [email protected] 1
Programa de Pós‑Graduação em Botânica, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, Curitiba, PR 81530‑000, Brazil
Embrapa Florestas, Colombo, PR 83411‑000, Brazil
2
1995). Other important compounds are essential oils, found in the leaves, flowers and fruits, and pigments in the seeds, which are exclusive to Campomanesia genus (Cardoso et al. 2010). This species is not currently cultivat
Data Loading...
