MicroRNA Detection and Target Identification Methods and Protocols
This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ h
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Tamas Dalmay Editor
MicroRNA Detection and Target Identification Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
MicroRNA Detection and Target Identification Methods and Protocols
Edited by
Tamas Dalmay School of Biological Sciences, University of East Anglia, Norwich, UK
Editor Tamas Dalmay School of Biological Sciences University of East Anglia Norwich, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6864-0 ISBN 978-1-4939-6866-4 (eBook) DOI 10.1007/978-1-4939-6866-4 Library of Congress Control Number: 2017937361 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface This book is a follow-up of a previous book in this series; therefore, it is unnecessary to introduce microRNAs (miRNAs) to any reader who is reading this preface. The previous book (MicroRNAs in development; published in 2011) described protocols to detect, profile, and manipulate miRNAs in various organisms, as well as how to validate targets of miRNAs in plants and animals. However, a lot of new techniques have been developed in the last 5–6 years, which warranted a new book. Some of the new protocols describe slight but important changes to well-established techniques that were described in the previous edition, such as Northern blot (Chapter 1) and preparation of cDNA libraries of small RNAs (Chapter 4). An alternative method to these two approaches to detect miRNAs is RT
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