MicroRNA Target Identification Methods and Protocols
This detailed book provides a comprehensive state-of-the-art presentation of all aspects of miRNA target identification, from the prediction of miRNA binding sites on their target molecules to their experimental validation and downstream functional analys
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Alessandro Laganà Editor
MicroRNA Target Identification Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
MicroRNA Target Identification Methods and Protocols
Edited by
Alessandro Laganà Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY, USA
Editor Alessandro Lagana` Department of Genetics and Genomic Sciences Mount Sinai School of Medicine New York, NY, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9206-5 ISBN 978-1-4939-9207-2 (eBook) https://doi.org/10.1007/978-1-4939-9207-2 Library of Congress Control Number: 2019936006 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface MicroRNAs (miRNAs) are small endogenous RNA molecules that regulate gene expression at the post-transcriptional level. Since their discovery in nematodes 25 years ago, extensive molecular, clinical, and computational research has uncovered their fundamental role in a plethora of biological processes and pathways, their crucial involvement in disease initiation and progression, and their implications as biomarkers for prognosis and drug response. miRNAs exert their function by binding target messenger RNAs (mRNA) through sequence complementarity and, consequently, inhibiting their translation or promoting their degradation. Thus, a fundamental task in miRNA research is the reliable identification of miRNA targets. Several compu
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