Two-Stage Pretreatment Process Validation for Production of Ethanol from Cotton Gin Trash
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Two-Stage Pretreatment Process Validation for Production of Ethanol from Cotton Gin Trash T. Vancov 1,2
&
J. Palmer 1 & B. Keen 1
# Crown 2019
Abstract This study reports on the evaluation of a two-stage pretreatment process for preparing cotton gin trash (CGT) for conversation to ethanol. During the first stage, CGT was mixed with acid at 12% H2SO4 on solids and heated to 180 °C for 15 min in a pressurised stirred reactor. Pressed first-stage pretreated fibres were heated to 200 °C for 5 min during the second stage. The two-stage process facilitated excellent sugar recovery from both cellulose (84%) and hemicellulose (78%) fractions of CGT. Recombinant yeast GSF335 propagated on first-stage liquors yielded 41.8 g kg−1 of dry CGT and was compared with the commercial yeast during separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentations (SSF). SHF of pretreated CGT fibre yielded the equivalent of 66.2 and 64.0 kg ethanol per tonne of unprocessed CGT with GSF335 and the commercial yeast, respectively. SSF ethanol yields for the commercial yeast were significantly lower (50.3 kg) while GSF335 correspondingly produced 63.8 kg ethanol per tonne of raw CGT. Keywords Cotton gin trash . Ethanol fermentation . Two-stage pretreatment . C5 yeast propagation
Introduction In Australia, cotton is a significant broad acre crop with more than half a million hectares currently under cultivation. It has been estimated that the ginning process in Australia generates somewhere between 25 and 60 kg of cotton gin trash (CGT) per bale of cotton; therefore, based on the 2017/2018 production statistics, up to 280,000 tonnes of CGT is projected [1, 2]. The high concentration of CGT residues poses a significant burden to the industry since the conventional practice of burning trash has ceased. The current practices for managing CGT varies greatly across the industry but for most, handling, storage, transport and disposal options add considerable cost to cotton-ginning processes [1]. Owing to increased disposal fees, stricter regulations on environmental applications and emissions and higher fuel prices, interest in converting this waste residue into bioenergy, particularly biofuels, is gaining attention. * T. Vancov [email protected] 1
NSW Department of Primary Industries, Wollongbar Primary Industries Institute, Wollongbar, NSW, Australia
2
Industry & Investment NSW, 1243 Bruxner Highway, Wollongbar, NSW 2477, Australia
CGT has been examined by several researchers as an exploitable biomass resource, particularly as a renewable feedstock in reinforcing commercial bioenergy purposes [3]. For instance, it has promising compositional attributes for effective conversion to biofuels and other valuable products relative to other candidate biomass feedstocks [4]. In biochemical conversion-based approaches, high yields of fermentable sugars have been generated from CGT and shown to support cellulosic ethanol production systems [5–7]. CGT is an ideal biorefinery feedstock because it is concentrat
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