miRNA-126-3p carried by human umbilical cord mesenchymal stem cell enhances endothelial function through exosome-mediate
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RESEARCH
Open Access
miRNA-126-3p carried by human umbilical cord mesenchymal stem cell enhances endothelial function through exosomemediated mechanisms in vitro and attenuates vein graft neointimal formation in vivo Qingxi Qu1, Limei Wang1, Weidong Bing2, Yanwen Bi2, Chunmei Zhang3, Xuanxuan Jing4 and Linghong Liu5,6*
Abstract Background: The aim of this study was to determine whether the combination of MSC implantation with miRNA126-3p overexpression would further improve the surgical results after vein grafting. Methods: human umbilical cord MSCs (hucMSCs) and human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords and characterized by a series of experiments. Lentivirus vector encoding miRNA-126-3p was transfected into hucMSCs and verified by PCR. We analyzed the miRNA-126-3p-hucMSC function in vascular endothelial cells by using a series of co-culture experiments. miRNA-126-3p-hucMSCs-exosomes were separated from cell culture supernatants and identified by WB and TEM. We validated the role of miRNA-126-3phucMSCs-exosomes on HUVECs proliferative and migratory and angiogenic activities by using a series of function experiments. We further performed co-culture experiments to detect downstream target genes and signaling pathways of miRNA-126-3p-hucMSCs in HUVECs. We established a rat vein grafting model, CM-Dil-labeled hucMSCs were injected intravenously into rats, and the transplanted cells homing to the vein grafts were detected by fluorescent microscopy. We performed historical and immunohistochemical experiments to exam miRNA-126-3phucMSC transplantation on vein graft neointimal formation and reendothelialization in vitro. (Continued on next page)
* Correspondence: [email protected] 5 Laboratory of Cryomedicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, People’s Republic of China 6 Research Center of Stem Cell and Regenerative Medicine, Shandong University, Jinan 250012, Shandong, People’s Republic of China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the d
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