Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels
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Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels José Javier Martín de Llano1, Graciela Fuertes1, Isabel Torró2, Consuelo García Vicent2, José Luis Fayos2 and Empar Lurbe*2 Address: 1Laboratory of the Pediatric Cardiovascular Risk Unit, Pediatric Department, Consorcio Hospital General Universitario de Valencia, and CIBER Fisiopatología de la Obesidad y Nutrición (Instituto de Salud Carlos III), Spain and 2Clinic of the Pediatric Cardiovascular Risk Unit, Pediatric Department, Consorcio Hospital General Universitario de Valencia, and CIBER Fisiopatología de la Obesidad y Nutrición (Instituto de Salud Carlos III), Spain Email: José Javier Martín de Llano - [email protected]; Graciela Fuertes - [email protected]; Isabel Torró - [email protected]; Consuelo García Vicent - [email protected]; José Luis Fayos - [email protected]; Empar Lurbe* - [email protected] * Corresponding author
Published: 24 April 2009 Journal of Translational Medicine 2009, 7:30
doi:10.1186/1479-5876-7-30
Received: 5 December 2008 Accepted: 24 April 2009
This article is available from: http://www.translational-medicine.com/content/7/1/30 © 2009 Martín de Llano et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Low birth weight has been related to an increased risk for developing high blood pressure in adult life. The molecular and cellular analysis of umbilical cord artery and vein may provide information about the early vascular characteristics of an individual. We have assessed several phenotype characteristics of the four vascular cell types derived from human umbilical cords of newborns with different birth weight. Further follow-up studies could show the association of those vascular properties with infancy and adulthood blood pressure. Methods: Endothelial and smooth muscle cell cultures were obtained from umbilical cords from two groups of newborns of birth weight less than 2.8 kg or higher than 3.5 kg. The expression of specific endothelial cell markers (von Willebrand factor, CD31, and the binding and internalization of acetylated low-density lipoprotein) and the smooth muscle cell specific α-actin have been evaluated. Cell culture viability, proliferation kinetic, growth fraction (expression of Ki67) and percentage of senescent cells (detection of β-galactosidase activity at pH 6.0) have been determined. Endothelial cell projection area was determined by morphometric analysis of cell cultures after CD31 immunodetection. Results: The highest variation was found in cell density at the confluence of endothelial cell cultures derived from umbilical cord arteries (66,789 ± 5,093 cells/cm2 vs. 45,630 ± 11,927 cells/ cm2, p < 0.05). Morphometric analysis
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