miRNA-23b as a biomarker of culture-positive neonatal sepsis
- PDF / 881,016 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 97 Downloads / 203 Views
Molecular Medicine
SHORT REPORT
Open Access
miRNA-23b as a biomarker of culturepositive neonatal sepsis Ahlam Fatmi1, Sid Ahmed Rebiahi2, Nafissa Chabni3, Hanane Zerrouki2, Hafsa Azzaoui1, Yamina Elhabiri2, Souheila Benmassour4, José Santiago Ibáñez-Cabellos5,6,7, Mohammed Chems-Eddine Smahi4, Mourad Aribi1, José Luis García-Giménez5,6,7 and Federico V. Pallardó5,6,7*
Abstract Background: Neonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking. Methods: Forty-eight newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1–2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR. Results: miR-23b levels increased in premature and full-term newborns in early onset sepsis (p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in full-term neonates (p < 0.05) compared to the respective negative controls. miR-23b levels also increased in late sepsis in the negative versus early sepsis negative controls (p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types (p < 0.0001 and p < 0.05 respectively). In early sepsis, miR-23b and death strongly and negatively correlated (correlation coefficient = − 0.96, p = 0.0019). In late sepsis, miRNA-23b and number of survivors (correlation coefficient = 0.70, p = 0.506) positively correlated. Conclusions: Lowering miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in molecular diagnosis and patient monitoring. Keywords: Early-onset sepsis, Haemoculture, Late-onset sepsis, miR-23b, Newborns
Introduction During the neonatal period, the immune system is still immature, and most immune responses are ensured by innate immunity, triggered following intimate contact between immune cells and microbes. In newborns, altered microbiota or microbial deprivation, as well as * Correspondence: [email protected] Mourad Aribi is senior author. 5 Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain 6 INCLIVA Health Research Institute, Mixed Unit for rare diseases INCLIVA-CIPF, Valencia, Spain Full list of author information is available at the end of the article
reduced microbial diversity, greatly increase the risk of immune dysregulation and proneness to inflammatory diseases. This makes neonates very fragile and more sensitive to several infectious diseases (Kumar and Bhat 2016; Lucignano et al. 2011). Nowadays, neonatal sepsis is one of the most dangerous conditions t
Data Loading...
