Mitogenome of Tolypocladium guangdongense
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GENOMICS, TRANSCRIPTOMICS, PROTEOMICS
Mitogenome of Tolypocladium guangdongense Chenghua Zhang 1 & Yongdong Dai 2 & Gangzheng Wang 1 & Chaoqun Wang 1 & Yu Gao 1,3 & Wangqiu Deng 1 & Taihui Li 1 Received: 4 July 2020 / Revised: 27 August 2020 / Accepted: 4 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Tolypocladium guangdongense is a high-value edible fungus with various medicinal and food safety properties. However, its evolutionary and genetic information is still limited. Mitochondrial genomes are potential models for molecular evolution and phylogenetic studies. In this study, we sequenced the complete mitogenome of T. guangdongense, demonstrating circular sequence of 46,102 bp, containing 14 standard protein-coding genes (PCGs), 2 ribosomal RNA subunit genes, and 28 tRNA genes. Phylogenetic analysis based on mitochondrial genes indicated that T. guangdongense was clustered into the Tolypocladium genus with high support value, based on the core PCG dataset. In addition, rps3 is also a suitable marker in the phylogenetic analysis in Hypocreales. Gene rearrangement analysis indicated that the gene order of PCGs was highly consistent in Hypocreales, and tRNA rearrangement events occurred in most species of Hypocreales; however, the rearrangement rates were not taxonomically correlated. Divergence time estimation based on the old fossil record and previous reports revealed that T. guangdongense originated approximately in the middle Cenozoic (42 Mya, 95% highest posterior density interval: 43–116) with the Tolypocladium genus differentiation. Our results provided more mitogenomic information of T. guangdongense and shed new insights into evolution of the Tolypocladium genus. Key points • The general and unique features of T. guangdongense mitogenome are firstly reported. • Phylogenetic analysis further verified the taxonomic status of T. guangdongense. • Divergence time estimation provides more evolutionary information of T. guangdongense. Keywords Cordyceps . Mitochondrial genome . Phylogenetic analysis . Comparative genomics . Gene rearrangement . Divergence time
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10889-1) contains supplementary material, which is available to authorized users. * Wangqiu Deng [email protected] * Taihui Li [email protected] 1
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China
2
Morphological Laboratory, School of Basic Medical Science, Guizhou University of Traditional Chinese Medicine, Guiyang, China
3
Hunan Agricultural University, Changsha, China
Compared with nuclear genomes, mitogenomes have several valuable characteristics including small size, rapid evolutionary rate, relatively conserved gene content and organization, maternal inheri
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