Molecular characterization of a novel cytorhabdovirus associated with paper mulberry mosaic disease
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Molecular characterization of a novel cytorhabdovirus associated with paper mulberry mosaic disease Yuanjian Qiu1,2 · Song Zhang1,2 · Jingjing Jin1,2 · Jiaxi Xie1,2 · Yumei Cao1,2 · Mengji Cao1,2 Received: 31 December 2019 / Accepted: 22 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel cytorhabdovirus, tentatively named “paper mulberry mosaic-associated virus” (PMuMaV), was discovered and identified by transcriptome sequencing (RNA-seq), small RNA sequencing (sRNA-seq), and RT-PCR amplification. The whole-genome sequence of PMuMaV is 13,736 nucleotides (nt) in length and contains six open reading frames (ORFs) encoding a nucleocapsid protein (N), a phosphoprotein (P), a putative movement protein (P3), a matrix protein (M), a glycoprotein (G), and an RNA-dependent RNA polymerase (L). The coding sequences are flanked by a 194-nt leader and a 370-nt trailer sequence at the 3’ terminus and 5’ terminus, respectively. Pairwise sequence comparisons showed that PMuMaV is related to northern cereal mosaic virus (NCMV, 38.97%), barley yellow striate mosaic virus (BYSMV, 38.86%), and maize yellow striate virus (MYSV, 38.76%), and phylogenetic analysis also placed these viruses together into the same branch, thus suggesting that PMuMaV is a member of a new species in the genus Cytorhabdovirus. Rhabdoviruses (family Rhabdoviridae), with the bulletshaped virions, are negative-sense single-stranded RNA viruses that infect humans, animals, and plants [1]. This family contains 30 genera, six of them are plant rhabdoviruses: Cytorhabdovirus (monopartite), Alphanucleorhabdovirus (monopartite), Betanucleorhabdovirus (monopartite), Gammanucleorhabdovirus (monopartite), Dichorhavirus (bipartite), and Varicosavirus (bipartite) [2–4]. Plant rhabdoviruses infect both monocot and dicot plants, including crops such as rice, wheat, maize, eggplant, and Chinese prickly ash [5]. Alphanucleorhabdoviruses, betanucleorhabdoviruses,
Handling Editor: Stephen John Wylie. Yuanjian Qiu and Song Zhang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04786-1) contains supplementary material, which is available to authorized users. * Mengji Cao [email protected] 1
National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing, China
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China
2
gammanucleorhabdoviruses, and possibly dichorhaviruses replicate in the nucleus, whereas cytorhabdoviruses replicate in the cytoplasm [5]. Cytorhabdoviruses are transmitted by aphids, planthoppers, and leafhoppers [2]. Their genomes (12.8-14.5 kb in size) encode six (lettuce yellow mottle virus, LNYV) to 10 (barley yellow striate mosaic virus, BYSMV) proteins, which invariably contain six conserved proteins in the order 3’–nucleocapsid protein (N)–phosp
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