Molecular characterization of an emerging reassortant mammalian orthoreovirus in China
- PDF / 707,382 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 105 Downloads / 185 Views
BRIEF REPORT
Molecular characterization of an emerging reassortant mammalian orthoreovirus in China Dandan Ye1 · Zhaoyang Ji1 · Hongyan Shi1 · Jianfei Chen1 · Da Shi1 · Liyan Cao1 · Jianbo Liu1 · Mingwei Li1 · Hui Dong1 · Zhaoyang Jing1 · Xiaobo Wang1 · Qiuge Liu1 · Qianjin Fan1 · Guangyi Cong1 · Jiyu Zhang1 · Yuru Han1 · Jiyong Zhou2 · Jinyan Gu2 · Xin Zhang1 · Li Feng1 Received: 1 April 2020 / Accepted: 22 May 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Mammalian orthoreoviruses (MRVs) infect almost all mammals, and there are some reports on MRVs in China. In this study, a novel strain was identified, which was designated as HLJYC2017. The results of genetic analysis showed that MRV HLJYC2017 is a reassortant strain. According to biological information analysis, different serotypes of MRV contain specific amino acid insertions and deletions in the σ1 protein. Neutralizing antibody epitope analysis revealed partial cross-protection among MRV1, MRV2, and MRV3 isolates from China. L3 gene recombination in MRV was identified for the first time in this study. The results of this study provide valuable information on MRV reassortment and evolution. Mammalian orthoreoviruses (MRVs) belong to the genus Orthoreovirus in the family Reoviridae. MRVs are nonenveloped, double-stranded (ds) RNA viruses that cause symptomatic or asymptomatic infection in mammals [1]. They are classified into four serotypes: MRV serotype 1 (MRV1) (Lang, T1L), MRV serotype 2 (MRV2) (Jones, T2J), MRV serotype 3 (MRV3) (Dearing, T3D), and MRV serotype 4 (MRV4) (Ndelle, T4N) [2]. The genome of MRVs Handling Editor: William G. Dundon. Dandan Ye and Zhaoyang Ji contributed equally to this paper. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04712-5) contains supplementary material, which is available to authorized users. * Jinyan Gu [email protected] * Xin Zhang [email protected] * Li Feng [email protected]; [email protected] 1
Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nangang District, Harbin 150001, China
Key Laboratory of Animal Virology of Ministry of Agriculture, Department of Veterinary Medicine, Zhejiang University, Hangzhou, China
2
is approximately 23,500 bp in total and contains 10 segments: three large (L) segments (L1-L3), three medium (M) segments (M1-M3), and four small (S) segments (S1-S4) [3]. Segments L1-L3 encode the λ3, λ2, and λ1 protein, respectively; segments M1-M3 encode the μ2, μ1, and μNS protein, respectively; and segments S1-S4 encode the σ1, σ2, σNS, and σ3 protein, respectively. Neutralization and hemagglutinin activities are restricted to the σ1 protein, which is located on the outer capsid of the virion [4]. The σ1 protein is responsible for attachment of the virus to the cell receptor and is used for serotype determination of MRVs [5]. MRVs are considered to be nonpathogen
Data Loading...
