Molecular cloning, characterization, and antioxidant function of catalase in Lymantria dispar asiatic (Lepidoptera: Lyma
- PDF / 678,628 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 35 Downloads / 165 Views
ORIGINAL PAPER
Molecular cloning, characterization, and antioxidant function of catalase in Lymantria dispar asiatic (Lepidoptera: Lymantriidae) under avermectin stress Jianyong Zeng1 • Bowen Zhang1 • Thi Minh Dien Vuong1,2 • Tingting Zhang1 Jing Yang1 • Guocai Zhang1
•
Received: 4 November 2018 / Accepted: 27 January 2019 Northeast Forestry University 2019
Abstract The critical antioxidant catalase (CAT) breaks down hydrogen peroxide induced by environmental stresses. Here we cloned full length catalase cDNA from Lymantria dispar asiatic (LdCAT). Bioinformatic analyses showed that open reading frames of LdCAT contains 1524 bp, encoding 507 amino acids with molecular weight of 126.99 kDa, theoretical pI of 5.00, aliphatic index of 29.92, grand average of hydropathicity of 0.764, and instability index (II) of 46.56. Protein BLAST and multiple sequence alignment indicated that LdCAT had high identity with CAT from other insects, especially lepidopterans. In a phylogenetic analysis, LdCAT was most similar to CAT from Spodoptera litura and S. exigua. Quantitative realtime polymerase chain reaction showed that LdCAT transcripts in all instar larvae and the five tissues tested, verifying the ubiquity of LdCAT in L. disapr. Moreover, LdCAT of third instar larvae was significantly upregulated
Project funding: This research was funded by the Fundamental Research Funds for Chinese Central Universities (Grant Nos. 2572017AA18 and 2572018AA09), the Chinese Central Financial Forest Science and Technology Extension Demonstration Fund Project (Grant No. JLT[2016]13). The online version is available at http://www.springerlink.com Corresponding editor: Ruihai Chai. Jianyong Zeng and Bowen Zhang contributed equally to this work. & Guocai Zhang [email protected] 1
School of Forest, Northeast Forestry University, Harbin 150040, People’s Republic of China
2
Vietnam Academy of Agricultural Sciences, Hanoi 100803, Vietnam
after they fed on avermectin at sublethal and LC10 doses. The highest relative transcript levels were found 2 h after an avermectin spray at LC90, and in the cuticula, rather than heads, fat bodies, malpighian tubes, and midguts after a spray avermectin at a sublethal concentration. The expression level of LdCAT under pesticide stresses here suggested that CAT is an important antioxidant enzyme of L. disapr defensing against pesticide stress and may be a good target for controlling this pest. Keywords Lymantria dispar asiatic Gypsy moth Catalase Molecular cloning Relative expression level Pesticide stress Instar
Introduction We recently reported that a mixture of avermectin and triflumuron increased efficacy against the destructive larvae of Lymantria dispar asiatic (gypsy moth) than either one alone (Zeng et al. 2018). Avermectin, a broad-spectrum insecticidal secondary metabolite of Streptomyces avermitilis against insects, mites and nematodes (Siddique et al. 2015; Xu et al. 2016; Mermans et al. 2017), acts on gamma-aminobutyric acid receptors (GABAr) and glutamate-gated chloride channel
Data Loading...
