Morpho-cultural and pathogenic variability among isolates of Stemphylium vesicarium (Wallr.) E. Simmons, causing Stemphy
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RESEARCH ARTICLE
Morpho‑cultural and pathogenic variability among isolates of Stemphylium vesicarium (Wallr.) E. Simmons, causing Stemphylium blight in onion collected from different geographical regions of Kashmir valley Mudasir Hassan1 · Vaseem Yousuf1 · Z. A. Bhat2 · N. A. Bhat1 · T. A. Shah1 · M. A. Khan3 · R. R. Mir3 · Roaf Ahmad Rather1 · Safoora Shafi3 Received: 21 July 2019 / Revised: 15 April 2020 / Accepted: 21 May 2020 © The Author(s) 2020
Abstract Stemphylium blight is the most destructive disease of onion crop and poses a grave threat to the very existence of its cultivation in Kashmir. Thirty six (36) isolates of Stemphylium vesicarium (Wallr.) E. Simmons were collected from different locations and characterized for cultural, morphological and pathogenic variations. Isolates produced velvety, cottony or fullfy colonies of different colours like whitish, light to dark grey, olivaceous with greenish tinge and brownish with filliform, entire and undulate margins. Significant variation in colony diameter and sporulation was observed among isolates. Mean hyphal width ranged from 3.11 to 5.48 µm. Conidiophore length varied from 20.07 to 92.56 µm. Similarly, mean conidiophore breadth varied from 2.84 to 7.58 µm. and were either light brown, light brown to brown, brown and dark brown in colour. Conidial colour of isolates varied from light brown, brown, light brown to brown and dark brown and are ovoid, ovoid to oblong and oblong in shape. Transverse septation varied from 0 to 6 and longitudinal from 0 to 5. Average ascus size of isolates varies from 103.74–204.24 × 23.00–33.11 µm. Average ascospore size varied from 12.08–41.96 × 10.06–17.38 µm among the isolates. Transverse and longitudinal septation varied from 3 to 7 and longitudinal from 0 to 6. Ascospores varied in colour from light brown, to dark brown. In shape the ascospores of different isolates were oblong with rounded base and conical apex, oblong with both ends rounded and ellipsoidal. Isolates exhibited variations in incubation period, number, size and colour of the lesions. Keywords Onion · Stemphylium blight · Stemphylium vesicarium · Variability · Pathogenicity
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42360-020-00253-8) contains supplementary material, which is available to authorized users. * Mudasir Hassan [email protected] 1
Division of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore, India
2
Division of Plant Pathology, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, India
3
Division of Genetics and Plant Breeding, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore, India
Onion (Allium cepa L.) is one of the most important and familiar crop throughout the world that belongs to the family Alliaceae. It is used as a common favo
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