Morpho-molecular variability and host reactivity of Albugo candida isolates infecting Brassica juncea genotypes in India
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ORIGINAL ARTICLE
Morpho-molecular variability and host reactivity of Albugo candida isolates infecting Brassica juncea genotypes in India Oinam Washington Singh 1 & Naveen Singh 2 & Deeba Kamil 1 & Vaibhav Kumar Singh 1 & Thokala Prameela Devi 1 & Lakshman Prasad 1 Received: 14 January 2020 / Accepted: 15 October 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract To generate information on variability in the pathogen causing white rust disease in Brassica species and their ability to infect Brassica juncea genotypes, thirteen isolates of Albugo candida infecting B. juncea and one isolate of Wilsonia bliti infecting Amaranthus blitum were collected from different rapeseed-mustard growing regions of India. These isolates were characterized based on different morphological data (sporangial shape and size; shape, size, and color of the pustule) and molecular data (cytochrome c oxidase subunit II (COX2) of mtDNA and internal transcribed spacer (ITS) region of rDNA). All the sporangia were globose or spherical to ellipsoidal with a size ranging from 199.01 μm2 to 357.23 μm2. White to creamy colored pustules were observed with a diameter ranging from 0.5–3.0 mm. Sequence analysis revealed that the Indian isolates of A. candida collected from B. juncea showed close identity with the Korean isolates infecting B. juncea. W. blilti isolate collected from A. blitum form a distinct clade with A. amaranthi infecting Amaranthus spinosus from Korea. Further, the pathogenic reaction of ten A. candida isolates was recorded on thirty genotypes of B. juncea that exhibited differential responses to the tested isolates. The three genotypes, PDZ-3, Pusa Karishma, and Donskaja were found to be resistant against all the ten isolates, which can further be used as a resistance source. PDZ-2 genotype was found to be susceptible only against the Ac-Hr isolate while the genotype BioYSR shows a susceptible reaction against Ac-Skn and Ac-Wltn isolates only. This set of thirty genotypes was able to differentiate the ten isolates of A. candida into four physiological races (AcRBj1 to AcRBj4) based on the disease reaction. The information generated through this study will be helpful for further study on identification and molecular mapping of resistance gene(s), their deployment over time and space, and, thus, better management of white rust disease in B. juncea. Keywords Genetic diversity . Races . Host differentials
Introduction Oilseed crops are the second most important determinant of the agricultural economy, next to cereals. Of these, Brassica species comprising B. rapa, B. juncea, B. napus, and B. carinata are one of the most important rabi oilseed crops. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s42161-02000690-4. * Lakshman Prasad [email protected] 1
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
2
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, Indi
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