Multilineage potential research of Beijing duck amniotic mesenchymal stem cells
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Multilineage potential research of Beijing duck amniotic mesenchymal stem cells Caiyun Ma . Kunfu Wang . Hongda Ji . Hongliang Wang . Liangcai Guo . Zhiyong Wang . Han Ren . Xishuai Wang . Weijun Guan
Received: 7 September 2017 / Accepted: 14 May 2018 Ó The Author(s) 2018
Abstract Amnion, which is usually discarded as medical waste, is considered as abundant sources for mesenchymal stem cells. In human and veterinary medicine, the multipotency of mesenchymal stem cells derived from amnion (AMSCs) together with their plasticity, self-renewal, low immunogenicity and nontumorigenicity characteristics make AMSCs a promising candidate cell for cell-based therapies and tissue engineering. However, up till now, the multipotential characteristics and therapeutic potential of AMSCs on preclinical studies remain uncertain. In this work, we successfully obtained AMSCs from Beijing duck embryos in vitro, and also attempted to detect their biological characteristics. The isolated AMSCs were phenotypically identified, the growth kinetics and karyotype were tested. Also, the cells were positive for MSCs-related markers (CD29, CD71,
CD105, CD166, Vimentin and Fibronection), while the expression of CD34 and CD45 were undetectable. Additionally, AMSCs also expressed the pluripotent marker gene OCT4. Particularly, when appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. Together, these results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine. Keywords Beijing duck Amniotic mesenchymal stem cells Multiply differentiation Biological characteristics
Introduction Caiyun Ma and Kunfu Wang have contributed equally to this work. C. Ma H. Ji H. Wang X. Wang W. Guan (&) Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China e-mail: [email protected] K. Wang College of Wildlife Resources, Northeast Forestry University, Harbin, China L. Guo Z. Wang H. Ren Mudanjiang Normal University, Mudanjiang 157011, China
To date, stem cell research has meant a tremendous advance for cell therapy and tissue engineering (Alizadeh et al. 2016; Bai et al. 2016; Gao et al. 2016; Guo et al. 2017; Zhang et al. 2016). Embryonic stem cells and adult type stem cells are current sources of stem cells(Gurel Pekozer et al. 2018; Kariminekoo et al. 2016; Mohammadian et al. 2016; Momenzadeh et al. 2017). However, given the ethical and technical problems, the use of embryonic stem cells may have obvious drawbacks, such as limited availability, complicated culture system and tumorigenicity (Blum and Benvenisty 2008; Gruen and Grabel 2006).
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Cell Tissue Bank
Acquisition of adult stem cells from bone marrow (BM-MSCs) is involved in invasive surgical manipulation, the number and self-renewal ability of BMMSCs significantly decreases with donor age (Gotherstrom et al. 2005). Expanding on this research, amnion (Bilic
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