NAC103, a NAC family transcription factor, regulates ABA response during seed germination and seedling growth in Arabido
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ORIGINAL ARTICLE
NAC103, a NAC family transcription factor, regulates ABA response during seed germination and seedling growth in Arabidopsis Ling Sun1 · Li‑Ping Liu1 · Ya‑Zhen Wang1 · Lei Yang1 · Mei‑Jing Wang2 · Jian‑Xiang Liu2 Received: 26 September 2020 / Accepted: 21 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion The Arabidopsis transcription factor NAC103 is up-regulated and its encoding protein is stabilized by ABA treatment, which positively regulates several ABA-responsive downstream genes during seed germination and seedlings growth. Abstract The Arabidopsis transcription factor NAC103 was previously found to be involved in endoplasmic reticulum (ER) stress and DNA damage responses. In this study, we report the new biological function of NAC103 in abscisic acid (ABA) response during seed germination and seedling growth in Arabidopsis. The expression of NAC103 was up-regulated and the NAC103 protein was stabilized by ABA treatment. Both the loss-of-function mutants of NAC103, created by targeted geneediting, and the over-expression plants of NAC103 have no obvious germination-related phenotype under normal growth conditions. However, under exogenous ABA treatment conditions, the NAC103 mutants were less sensitive to ABA during seed germination; in contrast, the NAC103 over-expression plants were more sensitive to ABA during seed germination and young seedling growth. Further, NAC103 regulated several ABA-responsive downstream genes including MYB78, MYB3, PLP3, AMY1, and RGL2. These results demonstrate that NAC103 positively regulates ABA response in Arabidopsis. Keywords NAC103 · ABA response · Seed germination · Seedling growth
Introduction The NAC (NAM, ATAF1,2, and CUC2) family is one of the largest gene families in plants genome (Olsen et al. 2005). Totally there are 117 NAC transcription factors in Arabidopsis, which are involved in diverse developmental Communicated by Fuminori Takahashi. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03502-2) contains supplementary material, which is available to authorized users. * Ling Sun [email protected] * Mei‑Jing Wang [email protected] 1
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China
2
programs and environmental stress responses (ShahnejatBushehri et al. 2016). Recently, NAC transcription factors have been found to play important roles in response to endoplasmic reticulum (ER) stress, in which the transcription factor NAC103, and the membrane-associated transcription factors NAC089 and NAC062 are all up-regulated by ER stress. NAC062 relocates from the plasma membrane to the nucleus and regulates the expression of ER stress-responsive genes (Yang et al. 2014a), and NAC089 relocates from the ER membrane to the nucleus to induce programmed cell death
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