Network Pharmacology Analysis to Uncover the Potential Mechanisms of Lycium barbarum on Colorectal Cancer
- PDF / 2,304,897 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 186 Views
ORIGINAL RESEARCH ARTICLE
Network Pharmacology Analysis to Uncover the Potential Mechanisms of Lycium barbarum on Colorectal Cancer Yi Lu1,2 · Jiachen Sun1,2 · Minhui Hu1,2 · Xianhe Kong1,2 · Weijie Zhong1,2 · Chujun Li1,2 Received: 10 February 2020 / Revised: 1 September 2020 / Accepted: 14 September 2020 © International Association of Scientists in the Interdisciplinary Areas 2020
Abstract Background Studies have shown that extracts from Lycium barbarum exerted protective effects against colorectal cancer (CRC) cells. We used the network pharmacology method to determine the effects of L. barbarum on CRC and to predict core targets, biological functions, pathways, and mechanisms of action. Method We obtained the active compounds and their targets in L. barbarum via use of the Traditional Chinese Medicine System Pharmacology Database (TCMSP), gathered the CRC targets from Malacards, TTD, GeneCards, and DisGeNET, and chosen the overlapped targets as the candidate targets. After protein–protein interaction (PPI) network analysis, 20 with the highest node degree were selected as the core targets, and their enrichment and pathways were analyzed. Furthermore, we employed iGEMDOCK to validate the compound-target relation. Result Eventually, 103 overlapped targets were chosen as the candidate targets. Targets with the top 20 highest node degree were selected as the core targets. Gene Ontology (GO) enrichment analysis indicated that the core targets were enriched in cell proliferation regulation, extracellular space, cytokine receptor binding, and so on. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis proved that the core targets were significantly enriched in bladder cancer, pathways in cancer. The docking results demonstrated that beta-sitosterol, glycitein, and quercetin had good binding activity to CRC putative targets. Conclusion Our work successfully predicted the functioning ingredients and potential targets of L. barbarum in CRC and illustrated the potential pathways and mechanisms comprehensively. Nevertheless, these results still call for in vitro and in vivo experiments to validate. Keywords Lycium barbarum · Colorectal cancer · Network pharmacology · GO · KEGG
1 Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12539-020-00397-1) contains supplementary material, which is available to authorized users. * Chujun Li [email protected] 1
Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, 26 Yuancun Erheng Road, Guangzhou 510655, People’s Republic of China
Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China
2
In the United States of America (USA) and in Asia, colorectal cancer (CRC) ranks as one of the top three most common cancers [1, 2]. Promotion of screening with increased use of advanced techniques have resulted in earlier detection of cancer, and
Data Loading...
