Cholesterol mediates spontaneous insertion of Lycium barbarum polysaccharides in biomembrane model
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Cholesterol mediates spontaneous insertion of Lycium barbarum polysaccharides in biomembrane model Ziyi Zhang1 · Changchun Hao1 · Hengyu Liu1 · Xianggang Zhang1 · Runguang Sun1 Received: 5 June 2019 / Revised: 23 July 2019 / Accepted: 15 November 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract In this work, the adsorptive behavior of Lycium barabrum polysaccharides (LBP) on 1-palmitoyl-2-oleoyl-sn-glycero3-phosphocholine(POPC)/1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and POPC/DPPC/Cholesterol (Chol) monolayer films were investigated using the Langmuir films technique and atomic force microscopy (AFM). For the POPC/DPPC system, the π-A isotherms shifted to larger areas and the surface pressure variations (Δπ) in the adsorption curves (10 mN/m and 35 mN/m) increased with the increase in the LBP concentration. For the POPC/DPPC/Chol system, π-A isotherms moved to larger areas with the addition of LBP, and the Δπ values were also lower than those of the POPC/DPPC system. These were ascribed to hydrogen bond network formation between LBP and the lipids. The excess per molecular area (ΔAexc) and the excess Gibbs free energy of mixing ( ΔGExc ) revealed that the repulsion force was the interaction between POPC and mix DPPC molecules. Meanwhile, the repulsion force increased with the addition of LBP. The addition of cholesterol decreased the force. The interactions among molecules were also evaluated by the values of K (the speed of adsorption of LBP), Ap (the number of each lipid molecule combining with the polysaccharide molecules in the mixed monolayer), Γ (the surface excess concentration) and A (the area per molecule). As shown in AFM images, the morphology of the monolayer films also indicated that LBP was easily adsorbed onto the POPC/DPPC monolayer film via hydrogen bond and the weak Van der Waals interaction. In summary, cholesterol could affect spontaneous insertion of LBP into the POPC/DPPC monolayer films from phosphate buffer solution. Keywords Lycium barbarum polysaccharides · 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine · 1,2-Dipalmitoyl-snglycero-3-phosphocholine · Cholesterol · Atomic force microscopy
1 Introduction Natural biopolymers offer many health benefits, and they can also be used in the treatment of disease. Lycium barbarum, as a type of natural biopolymer, has been investigated in the fields of medicine and food (Ahn et al. 2014). In particular, Lycium barbarum polysaccharides (LBP) are nontoxic, and they possess high pharmacological activity, including anti-aging, anti-tumor properties, antiviral, anti-radiation
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10450-019-00180-9) contains supplementary material, which is available to authorized users. * Changchun Hao [email protected] 1
School of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China
(Lee et al. 2014; Qian et al. 2017; Cheng et al. 2014; Bo et al. 2017; Zhao et al. 2015; Xing et al. 20
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