New insights into the toxic interactions of polyvinyl chloride microplastics with bovine serum albumin
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RESEARCH ARTICLE
New insights into the toxic interactions of polyvinyl chloride microplastics with bovine serum albumin Peng Ju 1,2 & Yu Zhang 1 & Jinfeng Ding 1 & Yifan Zheng 1 & Shuai Wang 1 & Fenghua Jiang 1 & Chengjun Sun 1,3 Received: 6 May 2020 / Accepted: 1 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The binding interaction between emerging pollutant polyvinyl chloride microplastics (PVC MPs) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, resonance scattering spectroscopy (RLS), UV-visible (UV-vis) absorption spectroscopy, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy under simulative physiological conditions. Fluorescence results revealed that the fluorescence quenching of BSA induced by PVC MPs was originated from the formation of BSA-PVC complex in static quenching mode. According to Stern-Volmer equation, the binding constants (Ka) between PVC MPs and BSA at different temperatures were obtained, and the number of binding sites was 1.62. The thermodynamic parameters, enthalpy change (ΔH), entropy change (ΔS), and free energy change (ΔG) were calculated to be − 41.77 kJ mol-1, 43.17 J mol-1 K-1, and − 54.63 kJ mol-1 via Van’t Hoff equation, indicating electrostatic interaction played a key role in the formation of BSA-PVC complex spontaneously. In addition, the alterations of microenvironment and secondary structure in BSA induced by PVC MPs were further confirmed by synchronous fluorescence spectra, UV-vis, FT-IR, and CD. This work not only provides further information for better understanding the binding interaction of PVC MPs with BSA, but also elucidates the potential biological toxicity of MPs at a molecular level. Keywords PVC . BSA . Microplastics . Fluorescence quenching . Interaction
Introduction Peng Ju and Yu Zhang contributed equally to this work. Responsible Editor: Lotfi Aleya Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11356-020-10707-1) contains supplementary material, which is available to authorized users. * Peng Ju [email protected] * Chengjun Sun [email protected] 1
Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, 6 Xianxialing Road, Qingdao 266061, People’s Republic of China
2
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), 1 Wenhai Road, Qingdao 266237, People’s Republic of China
3
Laboratory of Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), 1 Wenhai Road, Qingdao 266237, People’s Republic of China
Nowadays, plastics have appeared in every aspect of our everyday life because of their favorable properties including versatile, lightweight, malleable, durable, and low cost (Cozar et al. 2014; Law and Thompson 2014). However, the widespread use of plastics leads to a drama
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