New Trends in Fluorescence Spectroscopy Applications to Chemical and

Fluorescence is more and more widely used as a tool of investigation, analysis, control and diagnosis in many fields relevant to physical, chemical, biological and medical sciences. New technologies con­ tinuously emerge thanks to the progress in the desi

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Methods and Applications

o. Wolfbeis

Editor-in-Chief

Springer Berlin Heidelberg New York Barcelona Hong Kong London Milan Paris Tokyo

Bernard Valeur· Jean-Claude Brochon {Eds.}

New Trends in Fluorescence Spectroscopy Applications to Chemical and Life Sciences

With 187 Figures and 39 Tables

Springer

Fluorescence spectroscopy, fluorescence imaging and fluorescent probes are indispensible tools in numerous fields of modern medicine and sdence, science, including molecular biology, biophysics, biochemistry, clinical diagnosis and analytical and environmental chemistry. Applications stretch from spectroscopy and sensor technology to microscopy and imaging, to single molecule detection, to the development of novel fluorescent probes, and to proteomics and moleeule genomics. The Springer Series on Fluorescence aims at publishing state-of-the-art articles that can serve as invaluable tools for both praetitioners practitioners and researchers being active in this highly interdisciplinary field. The carefully edited collection of papers in each volume will give coninterdisdplinary tinuous inspiration for new research and will point to exciting new trends.

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ISSN 1617-1306 ISBN 978-3-642-63214-3 ISBN 978-3-642-56853-4 (eBook) DOI 10.1007/978-3-642-56853-4 Library of Congress Cataloging-in-Publication Data New trends in fluorescence speetroscopy spectroscopy:: applications to chemical and life science / Bernard Valeur, Jean-Claude Brochon (eds.). bibliographical referenees references and index. p.cm. - (Springer series on fluoreseenee; p.em. fluorescence; 1), Includes bibliographieal ISBN 978-3-642-63214-3 1. Fluorescence spectroscopy. I. Valeur, Bernard, 1944- II. H. Brochon, Jean-Claude, 1944- III. IH. Series. QD96.F56 N48 2001 543'.08584-dc21 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its eurrent current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. http://www.springeLde © Springer-Verlag Berlin Heidelberg 2001 Originally published by Springer-Verlag Berlin Heidelberg New York in 2001 Softcover reprint ofthe hardcover 1st edtion 2001 The use of registered names, trademarks, ete. etc. in this publieation publication does not imply, even in the specific statement, that such names are exempt from the relevant protective laws absence of a specifie and regulations and therefore free for general use. Typesetting: Fotosatz-Service Köhler Kohler GmbH, Würzburg Wlirzburg Production editor: Christiane Messerschmidt, Rheinau Cover: design & production, Heidelberg Printed on acid-free pap