Applications of Dual-Color Confocal Fluorescence Spectroscopy in Biotechnology
Classical applications of fluorescence spectroscopy detect emission which is collected from comparatively large ensembles of fluorescent particles, i.e. the signal is averaged over space and time. In contrast to this, confocal fluorescence methods restric
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CHEMICAL PHYSICS
Springer-Verlag Berlin Heidelberg GmbH
Physics and Astronomy
ONLINE LlBRARY
http://www.springer.de/phys/
Springer Series in
CHEMICAL PHYSICS Series Editors: F. P. Schäfer
J. P. Toennies
W. Zinth
The purpose of this series is to provide comprehensive up-to-date monographs in both weH established disciplines and emerging research areas within the broad fields of chemical physics and physical chemistry. The books deal with both fundamental science and applications, and may have either a theoretical or an experimental emphasis. They are aimed primarily at researchers and graduate students in chemical physics and related fields. 63 Ultrafast Phenomena XI Editors: T. Elsaesser, J.G. Fujimoto, D.A. Wiersma, and W. Zinth 64 Asymptotic Methods in Quantum Mechanics Application to Atoms, Molecules and Nuclei By S.H. Patil and K.T. Tang 65 Fluorescence Correlation Spectroscopy Theory and Applications Editors: R. Rigler and E.S. Eison 66 Ultrafast Phenomena XII Editors: T. Elsaesser, S. Mukamel, M.M. Murnane, and N.F. Scherer 67 Single Moleeule Spectroscopy Nobel Conference Lectures Editors: R. Rigler, M. Orrit, T. Basche
Series homepage - http://www.springer.de/phys/books/chemical-physics/ Volumes 1-62 are listed at the end ofthe book
R. Rigler M. Orrit T. Basche
Single Moleeule Spectroscopy Nobel Conference Lectures With 179 Figures
i
Springer
Professor Dr. Rudolf Rigler Karolinska Institutet, Department of Medical Biophysics, S-17177 Stockholm, Sweden e-mail: [email protected]
Dr. Michel Orrit CNRS et Universite Bordeaux I, C.P.M.O.H. 351 Cours de la Liberation, 33405 Talence, France e-mail: [email protected]
Professor Dr. Thomas Basche Johannes Gutenberg-Universität, Institut für Physikalische Chemie Jakob-Welder-Weg 11, 55099 Mainz, Germany e-mail: [email protected]
Series Editors: Professor EP. Schäfer
Professor W. Zinth
Max-Phinck-Institut für Biophysikalische Chemie D-37077 Göttingen-Nikolausberg, Germany
Universität München, Institut für Medizinische Optik Öttingerstr. 67 D-80538 München, Germany
Professor T.P. Toennies Max-Planck-Institut für Strömungsforschung Bunsenstrasse 10 D-37073 Göttingen, Germany
ISSN 0172-6218 ISBN 978-3-642-62702-6 Library of Congress Cataloging-in-Publication Data Single molecule spectroscopy : nobel conference lectures / [edited by1R. Rigler, M. Orrit, T. Basche. p. cm. - - (Springer series in chemical physics, ISSN 0172-6218 ; 67) ISBN 978-3-642-62702-6 ISBN 978-3-642-56544-1 (eBook) DOI 10.1007/978-3-642-56544-1 1. Molecular spectroscopy. I. Rigler, Rudolf. 11. Orrit, M. (Michel), 1956- III. Besche, T. IV. Springer series in chemical physics ; v. 67. QC454.M6 S57 2002 535.8'4- -dC21 2001049365 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or part
