Nitric Oxide Donor Regulated mRNA Expressions of LTC4 Synthesis Enzymes in Hepatic Ischemia Reperfusion Injury Rats
Cysteinyl LTs were associated with hepatic ischemia and reperfusion (I/R) injury. LTC4 synthesis enzymes including leukotriene C4 synthase (LTC4S), microsomal glutathione S-transferase (mGST) 2 and mGST3 can all conjugate LTA4 and reduced glutathione (GSH
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Nitric Oxide Donor Regulated mRNA Expressions of LTC4 Synthesis Enzymes in Hepatic Ischemia Reperfusion Injury Rats FF Hong, CS He, GL Tu, FX Guo, XB Chen and SL Yang Abstract Cysteinyl LTs were associated with hepatic ischemia and reperfusion (I/ R) injury. LTC4 synthesis enzymes including leukotriene C4 synthase (LTC4S), microsomal glutathione S-transferase (mGST) 2 and mGST3 can all conjugate LTA4 and reduced glutathione (GSH) to form LTC4, which involved hepatic ischemia–reperfusion (I/R) injury. This experiment was designed to further investigate the effects of V-PYRRO/NO (a selective liver nitric oxide donor)on the gene expressions of LTC4 synthesis enzymes during hepatic I/R. Adult male SD rats were divided into 3 groups: sham group (Control), I/R group and V-PYRRO/ NO ? I/R groups. Liver subjected to 1 h of partial hepatic ischemia followed by 5 h of reperfusion, saline or V-PYRRO/NO (1.06 lmol/kg/h) was administered intravenously through all the experimental periods. The mRNA levels of LTC4 synthesis enzymes in rat liver tissue were examined by RT-PCR. We observed that hepatic mRNA expressions of LTC4S and mGST3 were lower whereas mGST2 mRNA levels were higher in V-PYRRO/NO +I/R group than those in I/R group. Compared with control, only mGST3 mRNA was significantly declined in VPYRRO/NO +I/R groups. These results suggest that NO donor V-PYRRO/NO can downregulate the mRNA expressions of LTC4S and mGST3 but upregulate mGST2 mRNA expressions during hepatic I/R injury.
Keywords NO donor Microsomal glutathione S-transferase reperfusion injury Liver
Ischemia
F. Hong F. Guo X. Chen S. Yang (&) Department of Physiology, College of Medicine, Nanchang University, Nanchang 330031, China e-mail: [email protected] F. Hong G. Tu Department of Experimental Teaching, Nanchang University, Nanchang 330031, China C. He Health Supervision Station of Jinggangshan, Jinggangshan 343600 Jiangxi, China
S. Li et al. (eds.), Frontier and Future Development of Information Technology 3359 in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_435, Springer Science+Business Media Dordrecht 2014
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F. Hong et al.
435.1 Introduction Hepatic injury secondary to warm ischemia and reperfusion (I/R) is an important clinical issue. It has been implicated in the pathogenesis of a variety of clinical conditions including trauma, thermal injury, hypovolemic and endotoxin shock, reconstructive vascular surgery, liver transplantation, and liver resectional surgery. Hepatic I/R injuries occur during transplantation, liver resection for tumor, and circulatory shock [1]. The cysteinyl leukotriene (LT)s mediate a wide variety of biologic responses including enhanced vascular permeability, smooth muscle contraction, mucus hypersecretion, bronchial hyperreactivity [2]. Our previous studies showed cysteinyl LTs were associated with hepatic I/R injury [1, 3–5]. It is known that LTC4 synthesis enzymes including leukotriene C4 synthase (LTC4S), microsomal glutathione S-transfera
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