NMR resonance assignments of the programmed cell death protein 5 (PDCD5) from Toxoplasma gondii
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ARTICLE
NMR resonance assignments of the programmed cell death protein 5 (PDCD5) from Toxoplasma gondii Meng‑Hsuan Lin1 · Tsun‑Ai Yu2 · Chi‑Fon Chang3 · Yoshifumi Nishikawa4 · Chun‑Hua Hsu1,5,6 Received: 17 March 2020 / Accepted: 16 June 2020 © Springer Nature B.V. 2020
Abstract Toxoplasmosis is a systematic protozoan disease caused by a tiny parasite Toxoplasma gondii. The infection can be dangerous for pregnant woman and people with weak immune systems. The secreted protein named TgPDCD5 (Programmed cell death protein 5 from Toxoplasma gondii) plays an important role in apoptosis-inducing effect on host cells. Here, we report the 1H, 13C, and 15N resonance assignments of TgPDCD5. This work provides the ground for further structural elucidate and biophysical investigation about protein function. Keywords PDCD5 · Toxoplasma gondii · Apoptosis · NMR
Biological context Toxoplasma gondii (T. gondii), which lives as an obligate pathogenic protozoan parasite, can infect most of all warmblooded animals and has been a great threat to public health. Severe encephalitis and retinochoroiditis occur while T. gondii are attacking immunocompromised patients. Infection by T. gondii during pregnancy may cause abnormal pregnancy and fetal hydrocephalus (Maenz et al. 2014; Martin 2001). Only one standard treatment is used: combination of pyrimethamine, sulfadiazine (or clindamycine) and folinic acid. However, the parasite infecting to pregnant women, newborns and infants can’t be cured by standard treatment (Aleixo et al. 2016; Morjaria et al. 2016; Soheilian et al. * Chun‑Hua Hsu [email protected] 1
Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, Taiwan
2
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
3
Genomics Research Center, Academia Sinica, Taipei, Taiwan
4
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan
5
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan
6
Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan
2011). Because it is difficult to eliminate the T. gondii epidemic, the development of new drug against T. gondii is still urgent. Host majorly adapts their innate immunity to face the intracellular infection of T. gondii, therefore T. gondii has evolved to modulate the host innate immunity. This pathogen has also been discovered to regulate host cellular fate through apoptosis pathways (Brunet et al. 2008; Chang et al. 2015). While infecting fibroblasts and innate immune cells, T. gondii triggers host apoptosis to make penetration between tissues or immunity depression (Bannai et al. 2009; Gavrilescu and Denkers 2001). In the category of secreted proteins of T. gondii, programmed cell death protein 5 (TgPDCD5) has been discovered to induce apoptosis of human promyeloblastic cells and mouse macrophages (Bannai et al. 2008, 2009). Furthermore, by immuno-electron microscopic assay, TgPDCD5 was fo
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