Optimization of Submerged Culture for Exopolysaccharides Production by Morcella esculenta and its Antioxidant Activities
Morcella esculenta is an excellently edible and delicious morel mushroom found growing in China, Japan, and other Asian countries. Polysaccharides extracted from fungi have shown a variety of medical activities; however, the industrial large-scale product
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Optimization of Submerged Culture for Exopolysaccharides Production by Morcella esculenta and its Antioxidant Activities In Vitro Lihong Fu, Jinju Wang, Sheng Xu, Liming Hao and Yanping Wang Abstract Morcella esculenta is an excellently edible and delicious morel mushroom found growing in China, Japan, and other Asian countries. Polysaccharides extracted from fungi have shown a variety of medical activities; however, the industrial large-scale production of Morcella esculenta can not be achieved now due to the natural conditions. So this paper is concerned with optimization of submerged culture conditions for mycelial growth and exopolysaccharides (EPS) production of Morcella esculenta. by airlift fermentation. And the antioxidant activities of EPS were revealed by various free radical scavenging assays in vitro. The optimal medium constituents were determined as follows: (g/L): glucose 20 g/ L, peptone 4.5 g/L, KH2PO4 0.8 g/L, and MgSO47H2O 1.0 g/L. The optimum parameters of liquid culture were temperature 25 °C, cultivation time 5 d, rotary speed 140 rpm, and initial pH 6.0. This optimization strategy in shake flask culture leads to a mycelia yield of 4.93 ± 0.28 g/L, and EPS production of 1.65 ± 0.28 g/ L, respectively. Under optimal culture conditions, the maximum mycelia and EPS concentration in a 7 L airlift tower loop reactor were 6.13 g/L and 2.12 g/L for the first report, which were considerably higher than those obtained in preliminary studies that indicated the EPS production was closely correlated to the mycelia growth by Morcella esculenta. Futuremore, the EPS demonstrated positively antioxidant potential on 1,1-diphenyl-2-picrylhydrazyl radical scavenging, reducing power, and hydroxyl radical scavenging. These findings may provide the basis for the popular use of EPS in functional food or medicine. Keywords Morcella esculenta Antioxidant activity
Airlift fermentation
Exopolysaccharides
L. Fu J. Wang S. Xu Y. Wang (&) Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, People’s Republic of China e-mail: [email protected] L. Hao The Quartermaster Institute of the General Logistics Department of P.L.A, Beijing 100010, People’s Republic of China
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 249, DOI: 10.1007/978-3-642-37916-1_55, Ó Springer-Verlag Berlin Heidelberg 2014
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55.1 Introduction Morcella esculenta is a species of fungus in the Morchellaceae family of the Ascomycota. It is one of the most readily recognized of all the edible mushrooms and medical fungi [1]. Modern medical research shows that Morcella of fruiting body and mycelia has variety of medicinal activities, such as antioxidant, antivirus, anti-fatigue, immunomodulating, and anti-tumor [2–4]. However, commercial extract from the fruiting body of morel mushrooms has not been largely obtained till now. Submerged c
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