Oral microbial profile variation during canine ligature-induced peri-implantitis development
- PDF / 1,460,004 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 81 Downloads / 188 Views
RESEARCH ARTICLE
Open Access
Oral microbial profile variation during canine ligature-induced peri-implantitis development Shichong Qiao1†, Dongle Wu1†, Mengge Wang2, Shujiao Qian1, Yu Zhu1, Junyu Shi1, Yongjun Wei2* Hongchang Lai1*
and
Abstract Background: Dental implants have become well-established in oral rehabilitation for fully or partially edentulous patients. However, peri-implantitis often leads to the failure of dental implants. The aim of this study was to understand the core microbiome associated with peri-implantitis and evaluate potential peri-implantitis pathogens based on canine periimplantitis model. Results: In this study, three beagle dogs were used to build peri-implantitis models with ligature-induced strategy. The periimplant sulcular fluids were collected at four different phases based on disease severity during the peri-implantitis development. Microbial compositions during peri-implantitis development were monitored and evaluated. The microbes were presented with operational taxonomic unit (OTU) classified at 97% identity of the high-throughput 16S rRNA gene fragments. Microbial diversity and richness varied during peri-implantitis. At the phylum-level, Firmicutes decreased and Bacteroides increased during peri-implantitis development. At the genus-level, Peptostreptococcus decreased and Porphyromonas increased, suggesting peri-implantitis pathogens might be assigned to these two genera. Further specieslevel and co-occurrence network analyses identified several potential keystone species during peri-implantitis development, and some OTUs were potential peri-implantitis pathogens. Conclusion: In summary, canine peri-implantitis models help to identify several potential keystone peri-implantitis associated species. The canine model can give insight into human peri-implantitis associated microbiota. Keywords: Microbiota, Ligature-induced, Canine peri-implantitis, Microbial variation, Keystone taxonomy
Background With the development of oral technologies, dental implants are a highly successful and predictable treatment for replacing missing teeth, which can rehabilitate oral * Correspondence: [email protected]; [email protected] † Shichong Qiao and Dongle Wu contributed equally to this work. 2 Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education & School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, PR China 1 Department of Implant Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, PR China
health-related quality of life [1]. However, breakdown of soft and hard tissue around osseointegrated implants would result in the failure of dental implants known as inflammatory peri-implant disease [2–4]. Clinically, inflammatory peri-implant diseases are categorized into peri-implant mucositis and peri-implantiti
Data Loading...
